Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The insoluble fraction, SDS-I, obtained by boiling cell-walls of Brucella abortus in sodium dodecyl sulfate (SDS) was hydrolyzed by lysozyme. Three major bands of apparent molecular weight 37000 (I), 25000 (II), 15000 (III) were isolated by gel slicing after polyacrylamide gel electrophoresis in the presence of SDS. The three bands were injected subcutaneously to mice using Freund incomplete adjuvant and immunity was tested one month later by intraperitoneal administration of a virulent strain of B. abortus. Fifteen days after challenge, numbers of viable brucella within the spleens were determined. The isolated bands, I, II and III were able to protect mice at a level corresponding to the killed whole-cells Brucella melitensis H38 reference vaccine.
 ...
PMID:Isolation of three Brucella abortus cell-wall antigens protective in murine experimental brucellosis. 680 61

Ralston, Doris J. (University of California, Berkeley), B. S. Baer, and S. S. Elberg. Lysis of brucellae by the combined action of glycine and a lysozyme-like agent from rabbit monocytes. J. Bacteriol. 82:342-353. 1961.-An acid-extractable lytic material was obtained from rabbit monocytes. It acts on a substrate in the walls of brucellae and has properties similar to egg-white lysozyme. Brucella melitensis strain Rev Is is completely resistant to this agent and also to crystalline lysozyme, but when the cells are exposed to sufficient amounts of glycine, the surface is rendered susceptible to these lytic agents. Rough type Rev Is cells are more susceptible than smooth, and the virulent B. melitensis strain 6015 is most resistant.
 ...
PMID:Lysis of brucellae by the combined action of glycine and a lysozyme-like agent from rabbit monocytes. 1373 76