Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Experiments in vivo have shown that the ticks of Ornithodoros papillipes inhibit both Gram-positive and Gram-negative bacteria. On Gram-positive microorganisms most sensitive to bactericidal effect of ticks have turned to be micrococci, staphylococci, streptococci and bacteria of diphtheria, of Gram-negative ones--Bacillus coli. Of Gram-positive microorganisms less sensitive were Listeria, of Gram-negative ones--salmonellae, agents of tularemia and pseudotuberculosis. At the infection with bacteria the organism of the tick produces a bactericidal substance which causes the dying off of the microbal population. The ability of the bactericidal substance to lyse the inhibited cells of Micrococcus lysodeiktickus as well as the coincidence of data on sensibility to the tick bactericidal substance obtained experimentally in vitro and in vivo have shown that lysozyme is an active antibacterial substance in the tick's organism.
 ...
PMID:[Comparative study of the bactericidal action of the body of Ornithodoros papillipes ticks]. 10 Jul 56

Several methods for the isolation of plasmid DNA from Yersinia pseudotuberculosis have been tried; the aim of the study has been a differentiated isolation of low-molecular plasmids from natural strains of bacteria containing plasmids with various molecular masses. The modifications of the known methods have been developed, that rule out the use of lysozyme for cell lysis and permit prepare clarified lysates containing mostly low-molecular plasmid DNA from natural strains of bacteria containing high-molecular plasmids as well.
 ...
PMID:[Differential isolation of low-molecular plasmids]. 247 Sep 74

The in vitro and in vivo studies showed that lysozymes obtained from O. papillipes, O moubata, A. lahorensis and H. asiaticum had bacteriostatic and bactericidal effects. The effect of the lysozymes obtained from the above ticks was studied in comparison to that of the egg lysozyme. Micrococci, staphylococci, streptococci, E. coli and C. diphtheriae proved to be the most sensitive. Salmonella, Listeria, Pasteurella pseudotuberculosis and Pasteurella tularensis were less sensitive. The comparison of the antibacterial effects of the lysozymes obtained from the ticks and the egg lysozyme showed that the lysozyme from O. moubata, O. papillipes and A. lahorensis had the most pronounced bactericidal effect. The lysozyme obtained from H. asiaticum had the lowest bactericidal activity.
 ...
PMID:[Biological activity of lysozymes of various origins]. 714 82

The action of bactericidal polycationic peptides was compared in Yersinia spp. by testing peptide binding to live cells and changes in outer membrane (OM) morphology and permeability. Moreover, polycation interaction with LPS was studied by measuring the dependence of dansylcadaverine displacement and zeta potential on polycation concentration. When growth at 37 degrees C, Yersinia pestis and Yersinia pseudotuberculosis bound less polymyxin B (PMB) than pathogenic or non-pathogenic Yersinia enterocolitica, regardless of virulence plasmid expression. Y. pseudotuberculosis OMs were unharmed by PMB concentrations causing extensive OM blebbing in Y. enterocolitica. The permeability to lysozyme caused by PMB was greater in Y. enterocolitica than in Y. pseudotuberculosis or Y. pestis and differences increased at 37 degrees C. Similar observations were made with other polycations using a polymyxin/novobiocin permeability assay. With LPS of cells grown at 26 degrees C, polycation binding was highest for Y. pseudotuberculosis and lowest for Y. pestis, with Y. enterocolitica yielding intermediate results which were lower for pathogenic than for non-pathogenic strains. With LPS of cells grown at 37 degrees C, polycation binding remained unchanged for Y. pestis and pathogenic Y. enterocolitica, increased for non-pathogenic Y. enterocolitica and decreased for Y. pseudotuberculosis to Y. pestis levels. Polycation binding related in part to differences in charge density (zeta potential) of LPS aggregates, suggesting similar effects at bacterial surfaces. It is suggested that species and temperature differences in polycation resistance relate to infection route, invasiveness and intracellular multiplication of Yersinia spp.
 ...
PMID:Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica. 963 21

Molecular chaperones are involved in folding, oligomerization, transport, and degradation of numerous cellular proteins. Most of chaperones are heat-shock proteins (HSPs). A number of diseases of various organisms are accompanied by changes in the structure and functional activity of chaperones, thereby revealing their vital importance. One of the fundamental properties of chaperones is their ability to bind polypeptides lacking a rigid spatial structure. Here, we demonstrate that affinity chromatography using sorbents with covalently attached denatured proteins allows effective purification and quantitative assessment of their bound protein partners. Using pure Escherichia coli chaperone GroEL (Hsp60), the capacity of denatured pepsin or lysozyme-based affinity sorbents was evaluated as 1 mg and 1.4 mg of GroEL per 1 ml of sorbent, respectively. Cell lysates of bacteria (E. coli, Thermus thermophilus, and Yersinia pseudotuberculosis), archaea (Halorubrum lacusprofundi) as well as the lysate of rat liver mitochondria were analyzed using affinity carrier with denatured lysozyme. It was found that, apart from Hsp60, other proteins with a molecular weight of about 100, 50, 40, and 20 kDa are able to interact with denatured lysozyme.
 ...
PMID:Affinity chromatography of chaperones based on denatured proteins: Analysis of cell lysates of different origin. 2664 95

Literature data and results of authors' research on biological properties of corynebacteria of reproductive tract of women are analyzed. General characteristics of microorganisms is given. 20 species of corynebacteria are presented: C. amycolatum, C. aquaticum, C. aurimucosum, C. bovis, C. glucuronolyticum, C. coyleae, C.freneyi, C.jeikeium var. genitalium, C.jeikeium var. pseudogeni- talium, C. lipophiloflavum, C. kutscheri, C. minutissimum, C. nigricans, C. pseudodiphtheriticum, C. pseudotuberculosis, C. renale, C. striatum, C. tuberculostearicum (lipophile) (includes most CDC group G-2 strains), C. xerosis and C. urealyticum. Mechanisms and factors ensuring the ability of corynebacteria to exist in vaginal biotope regardless of microecological condition- the presence of high resistance to factors of innate immunity (lysozyme, complement, immunoglobulins), pH- dependent adhesion to fibronectin and vaginal epitheliocytes - are examined. The role of fi- bronectin in adhesion of bacteria to vaginal epithelial cells is described. Corynebacteria exome- tabolites are shown to facilitate maximal realization of antagonistic activity of vaginal peroxide-producing lactobacilliby supressing catalase ofopportunistic microorganisms-symbionts, that directly influences the quantity and structure of bacterial population by suppressing growth and biofilm-formation. The materials provided give evidence on the significant role of corynebac- teria in realization of physiological phenomenon - colonization resistance and allow us to exam- ine these microorganisms as an integral part of normal microbiota of woman reproduction tract.
 ...
PMID:[PHYSIOLOGICAL FEATURES OF CORYNEBACTERIA OF FEMALE REPRODUC- TIVE TRACT]. 3069 93