Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The distinctive mononuclear cells that appear in the cortical sinuses of lymph nodes in toxoplasma lymphadenitis and other conditions have been termed "immature sinus histiocytes," although these cells have neither enzyme histochemical nor ultrastructural features of histiocytes. The authors stained these cells in frozen sections of six reactive lymph nodes, using the immunoperoxidase technique, with monoclonal antibodies to T cell, B cell, and monocyte antigens and with heteroantisera to immunoglobulin and lysozyme. The immature sinus histiocytes stained with a monoclonal antibody to B cells (anti-B1) and had immunoglobulin of the IgG class. They did not react with monoclonal antibodies to T cells or monocytes or with antilysozyme. In contrast, medullary sinus histiocytes in three additional lymph nodes were B1-negative and reacted with anti-T4, anti-M1, and anti-lysozyme. These results indicate that immature sinus histiocytes are IgG-bearing B lymphocytes rather than histiocytes. Their role in the immune response remains enigmatic.
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PMID:Characterization of immature sinus histiocytes (monocytoid cells) in reactive lymph nodes by use of monoclonal antibodies. 654 24

Inflammatory cells in lymph nodes of eighteen patients suffering from culture-proven tuberculous lymphadenitis were examined by histological and immunohistochemical techniques. Ten patients suffered from symptomatic HIV-infection and eight patients were immunocompetent individuals without HIV-1 serology. Characteristic granulomas with or without caseation were observed in eight immunocompetent and four HIV-1-infected patients with less marked lymphopenia of CD4 positive peripheral blood lymphocytes. No epitheloid cell formation was present in lymph nodes of HIV1-infected patients with more severe depression of CD4 positive peripheral blood lymphocyte count. Foamy macrophages were found instead of these cells. While many cells--predominantly lymphocytes--express CD25 (IL-2 receptor) in cases with typical epitheloid granulomas there is no such CD25 expression in cases without any epitheloid cell formation. This result suggest that T cell function is necessary for epitheloid granuloma formation in human tuberculosis. The phenotype of macrophages underwent progressive changes parallel to decreasing numbers of CD4 positive peripheral blood lymphocytes. Foamy macrophages in Mycobacterium avium-intracellulare infection represented an end-stage phenotype. They were positive for S100 protein and they did not express lysozyme, alpha-1-anti-chymotrypsin, L1 antigen (Mac387) and CD4, whereas positivity for HLA-DR, CD68 and Ki-M8 was preserved. In situ immunohistochemical demonstration of IFN-alpha, IFN-beta, TNF-alpha, IL-1 and IL-6 revealed that foamy cells in M. tuberculosis infection were highly active effector cells. They contained higher concentrations of the examined cytokines than epitheloid cells in the lesions of HIV+ and HIV-patients. Corresponding to these findings the histological proof of acid-fast bacilli was generally not successful in typical HIV-associated tuberculosis. The foamy appearance may result from the lipid-rich cell membranes of destroyed acid-fast bacilli. In contrast acid-fast bacilli-packed foamy macrophages in AIDS patients with M. avium-intracellulare (MAI) infection did not produce any of the examined cytokines.
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PMID:Immunohistochemical analysis of cell composition and in situ cytokine expression in HIV- and non-HIV-associated tuberculous lymphadenitis. 771 49

Twenty-eight cases of non-overt necrotizing type Kikuchi-Fujimoto disease (KF disease, histiocytic necrotizing lymphadenitis) were investigated clinicopathologically, immunohistochemically and electron microscopically in order to analyze the nature of this disease. In addition, investigations to detect the presence of Epstein-Barr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization (ISH) were also performed as a high incidence of positive serum immunoreactivity to EBV had been revealed in the cases examined. The clinical features were an equal male to female ratio, mean age of 26 years, mild leukopenia and about 40% association with fever over 38 degrees C. The major particular pathologic features were: (i) varying amounts of nuclear debris in the pathologic areas, identified as apoptosis by electron microscopy; (ii) presence of medium to large sized transformed lymphocytes (immunoblasts), a very small number of them positive for both UCHL1 and L26, and plasmacytoid cells, some of them positive for UCHL1; and (iii) characteristically shaped histiocytes predominant in the pathologic areas, with irregular nuclei and strongly positive for anti-lysozyme and anti-alpha 1-antitrypsin antibodies. Investigations with PCR and ISH revealed a complete absence of EBV in these cases despite excellent results for positive controls. It was therefore considered that EBV was not a causative virus for KF disease.
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PMID:Pathologic analyses of non-overt necrotizing type Kikuchi and Fujimoto's disease. 831 Aug 24

An immunohistologic study of lymph nodes from 21 patients with Kikuchi's disease (histiocytic necrotizing lymphadenitis) was performed. The cell components of the affected areas were mainly CD4-positive cells, CD8-positive T cells, alpha/beta T-cell gene receptor-positive T cells, and lysozyme-staining cells. CD3-positive or alpha/beta T-cell gene receptor-positive T cells were composed mainly of CD8-positive and CD11b-negative cytotoxic T cells. Double staining demonstrated that CD4-positive cells usually were positive for Ki-M1p, a marker of plasmacytoid monocytes, but negative for T-cell markers. Although some lysozyme and CD4 double-positive cells were recognized, most CD4-positive cells were negative for lysozyme. The results indicate that CD4-positive cells in the affected foci of Kikuchi's disease were mainly composed of plasmacytoid monocytes.
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PMID:Immunohistologic studies of Kikuchi's disease. 840 21

Clinical course of acute nonspecific lymphadenitis of the face and neck is studied in 241 patients aged 1-14 years in order to improve the diagnosis and therapy of this condition. General clinical examinations were supplemented by laser biophotometry and assessment of the activities of some local defense factors (lysozyme, beta-lysine, secretory and serum IgA). Multiple-modality treatment for the first time included magnetic laser therapy (MLT), and its efficacy is assessed. Results of biophotometry helped define objective criteria for the diagnosis and evaluation of treatment efficacy in children with acute lymphadenitis of the face and neck. MLT proved to be a highly effective treatment modality, decreasing the inflammation and correcting local (oral) defense factors. Use of MLT accelerated all phases of the inflammatory process, promoted its regression at the serous stage, and decreased the incidence of suppurative forms of acute lymphadenitis by 40%.
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PMID:[Low-intensity infrared laser radiation in the diagnosis and combined treatment of acute nonspecific lymphadenitis of the face and neck in children]. 1022 5


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