Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The authors present materials for comparison of three methods of E. coli infection by the RNA of the MS2 bacteriophage. The cells accepted the RNA at the stage of spheroplasts (obtained with the aid of a lysozyme and EDTA) with the efficacy of up to 1.5 X 10(5) IC/mug of the RNA. Diethylpyrocarbonate was used as a transfection stimulant. The product of transfection was identical to the MS2 bacteriophage. The efficacy of transfection in the calcinated system and on the frozen-defrosted bacteria was lower than on the spheroplasts (10(2) IC/mug of the RNA) which was largely associated with the formation of "latent infectious centres". Formation of latent negative colonies was characteristic of bacteriophage MS2, but formation of these colonies was intensified when calcinated cells were used for transfection.
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PMID:[Transfection of Escherichia coli using bacteriophage MS2 RNA]. 76 35

The crystal structure of an antibacterial protein of immune origin (TSWAB), purified from tasar silkworm (Antheraea mylitta) larvae after induction by Escherichia coli infection, has been determined. This is the first insect lysozyme structure and represents induced lysozymes of innate immunity. The core structure of TSWAB is similar to c-type lysozymes and alpha-lactalbumins. However, TSWAB shows significant differences with respect to the other two proteins in the exposed loop regions. The catalytic residues in TSWAB are conserved with respect to the chicken lysozyme, indicating a common mechanism of action. However, differences in the noncatalytic residues in the substrate binding groove imply subtle differences in the specificity and the level of activity. Thus, conformational differences between TSWAB and chicken lysozyme exist, whereas functional mechanisms appear to be similar. On the other hand, alpha-lactalbumins and c-type lysozymes exhibit drastically different functions with conserved molecular conformation. It is evident that a common molecular scaffold is exploited in the three enzymes for apparently different physiological roles. It can be inferred on the basis of the structure-function comparison of these three proteins having common phylogenetic origin that the conformational changes in a protein are minimal during rapid evolution as compared with those in the normal course of evolution.
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PMID:Structure of the induced antibacterial protein from tasar silkworm, Antheraea mylitta. Implications to molecular evolution. 1152 83

The effects of treatment/prophylaxis of newborn calf colibacillosis with tryptic casein hydrolysate (TCH), recently shown to be a novel type of antimicrobial acting through stimulation of the microbial autolytic system, versus an authorized veterinary drug, Fermosorb, were evaluated. Both products showed similar high therapeutic and prophylactic efficacies, but hematological indices and daily weight gain of cured/protected animals were better with TCH. The differences in hemoglobin and hematocrit levels, total protein, gamma-globulin and sulfhydryl group quantities, bactericidal and lysozyme activities as well as daily weight gain at the end of treatment/prophylaxis were statistically significant (P<0.05-0.000005). Statistically significant differences (P<0.05-0.0005) in favor of TCH were also observed when bactericidal activity, total protein quantity of serum as well as daily weight gain of the animals were compared on the 90th day after birth. We conclude that TCH acts not only as an antimicrobial, but also as an immunostimulant (and growth promoter). The immunostimulatory activity of TCH most probably derives from a synergistic action of bioactive peptides encrypted in the preparation itself and the cell wall fragments resulting from microbial autolysis induction.
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PMID:Non-specific immunity-enhancing effects of tryptic casein hydrolysate versus Fermosorb for treatment/prophylaxis of newborn calf colibacillosis. 1462 99