Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The hypocotyl protoplasts of Lycium barbarum L. CV. Ningji No. 1 were isolated in an enzyme mixture solution containing 1% cellulase and 1% pectinase. The protoplasts were cultured in KM 8 p liquid medium containing 0.3 mg/L 2,4-D and 0.3 mg/L BA, The first division of regenerated cells occurred 3 days after culture. The small cell clumps could be observed by naked eyes 20 days after culture. 40 days after culture, microcalli of 1-2 mm in size were transferred to MS solid differentiation medium containing 3% sugar, 0.1-0.4 mg/L BA and 0.05-0.1 mg/L NAA. About 30 days after culture on MS solid medium, the calli with shoots were transferred to 1/2 MS medium containing 0.1 mg/L BA and 0.2 mg/L IBA, from which 80% of them differentiated roots and regenerated whole plantlets.
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PMID:[Plant regeneration from hypocotyl protoplasts of Lycium barbarum L]. 835 55

A study was undertaken to develop a protoplast regeneration system for pinellia. A yield of 19 29 x 10(5) protoplasts/g F. W. could be obtained from cell suspension cultures incubated in a digestion enzyme solution with 2% cellulase Onzuka R-10, 10% pectinase (Sigma), 0.01% pectolyase Y23. K8P and modified MS media were used to culture protoplasts in: a) liquid, b) liquid-solid double layer, or c) agarose embedded protoplast culture. The former two were conducive to colony formation from protoplast-derived cells. The frequency of cell division was about 8% after 3 days in culture. Gradually adding fresh medium of lower osmotic pressure into the medium for protoplast culture favored cell division. Calli (1-2 mm in diameter) formed after 30-40 days in culture. The calli transferred onto medium supplemented with KT (0.5 mg 1(-1)) and NAA (0.2 mg 1)(-1)) could regenerate plants after 40-50 days. Of 47 plantlets transplanted into plots, 29 flowered and were fertile.
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PMID:Protoplast culture and plant regeneration ofPinellia ternata. 2417 63