Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two experiments with young pigs (25 d of age) were conducted to investigate the effect of multienzyme preparations on nutrient digestibility, growth performance, and P utilization and excretion. In Exp. 1, 24 pigs (six pigs per treatment) were used in a 28-d performance and digestibility trial using four diets: control (no enzyme) and control supplemented with enzyme preparation A, B, or C. The control diet was formulated to meet 95% of NRC (1998) nutrient specifications (except for available P, which was at 44% NRC) and composed of corn, wheat, wheat by-products, barley, soybean meal, canola meal, and peas. All three enzyme preparations contained xylanase, glucanase, amylase, protease, invertase, and phytase activities and differed in the type of plant cell wall-degrading activities; Enzyme A contained cellulase, galactanase, and mannanase; Enzyme B contained cellulase and pectinase; and Enzyme C contained cellulase, galactanase, mannanase, and pectinase. Pigs fed enzyme-supplemented diets had higher ADG (P = 0.02) and G:F (P = 0.01) than those fed the control diet. On average, and when compared with control diet, enzyme supplementation improved (P = 0.001 to 0.04) ileal digestibility of DM (60 vs. 66%), GE (62.8 vs. 70.4%), CP (62 vs. 72%), starch (86.7 vs. 94.2%), nonstarch polysaccharides (NSP; 10.1 vs. 17.6%), and phytate (59 vs. 70%). Compared with the control, total-tract digestibility of nutrients was increased (P = 0.001 to 0.01) owing to enzyme supplementation, with Enzyme C showing the highest improvement in DM, GE, CP, starch, NSP, phytate, and P utilization. Pigs fed enzyme-supplemented diets had decreased (P = 0.04) fecal P excretion. The benefit from improved nutrient utilization with enzyme supplementation was further substantiated in a 38-d growth performance study with 48 pigs. The control and Enzyme C-supplemented diets (same as Exp. 1) were assigned to six replicate pens (four pigs per pen). The study was conducted in three phases (Phase 1 = d 0 to 7; Phase 2 = d 7 to 21; Phase 3 = d 21 to 38). Individual BW and pen feed disappearance were monitored. Average daily gain and G:F were 231 and 257 g (P = 0.01), and 0.56 and 0.63 (P = 0.001) for the control and enzyme-supplemented diets, respectively. It is evident from this study that the use of enzyme preparations may allow for cost-effective and environmentally friendly formulation of young pig diets.
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PMID:Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs. 1508 Mar 27

The effect of carbohydrase enzyme supplementation on energy utilization from full-fat canola seed was investigated in a TMEn assay with adult roosters and in a nutrient digestibility and growth performance study with broiler chickens. In the TMEn assay, enzyme preparations C (cellulase, 340 U/g), XG (xylanase, 63,600 U/g and glucanase, 48,300 U/g), P (pectinase, 10,000 U/g), and MC (mannanase, 10,900 U/g and cellulase, 600 U/ g), alone and in combination (C + P, C + XG, C + MC, P + XG, P + MC, XG + MC, C + P + XG, C + P + MC, and C + P + XG + MC), were evaluated at an inclusion level of 0.1%. On average, hammer-milled canola seed with a TMEn content of 3,642 kcal/kg showed an increase (P < 0.05) to 4,783 kcal/kg following supplementation with the enzyme blends C + P + XG, C + P + MC, and C + P + XG + MC. A similar pattern of increase (P < 0.05) in fat (80.4 vs. 63.5%) and nonstarch polysaccharide (NSP; 20.4 vs. 4.4%) digestibilities was observed. Enzyme combination C + P + XG was further evaluated in a 2-wk (5- to 18-d) trial with broiler chickens fed isonitrogenous and isoenergetic corn and soybean meal-based diets containing canola seed (15%), the corresponding canola meal (8.85%) plus canola oil (6.15%) mixture, or canola seed (15%) supplemented with 3 different levels (0.002, 0.01, or 0.05%) of the enzyme. Poorer (P < 0.05) feed:gain (1.412 vs. 1.344), lower (P < 0.05) total tract DM (65.9 vs. 70.7%) and fat (69.6 vs. 88.0%) digestibilities, lower AMEn content (2,963 vs. 3,200 kcal/kg), and lower ileal fat (65.6 vs. 85.6%) and protein (75.6 vs. 81.2%) digestibilities were observed for the canola seed diet compared with the canola meal plus canola oil diet. Enzyme supplementation of the canola seed diet resulted in an improvement (P < 0.05) in feed:gain; total tract DM, fat, and NSP digestibilities; AMEn content; and ileal fat digestibility. Although the enzyme effect on ileal and total tract fat digestibilities was significant at both high and medium inclusion levels, other parameters showed the significant improvement only when the highest inclusion rate of enzyme was used. These data support the need for carbohydrase enzyme supplements in poultry diets containing full-fat canola seed.
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PMID:The use of enzyme technology for improved energy utilization from full-fat oilseeds. Part I: canola seed. 1677 71