Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using the previously cloned Aspergillus niger N756 pectin lyase D gene as a probe, the corresponding pelD gene has been isolated from a genomic library of the laboratory strain A. niger N400. This gene encodes PLD, previously described as PLI, which is one of the two major pectin lyases isolated from the commercial pectinase preparation Ultrazym. Heterologous hybridization of the A. niger N400 genomic library with the pelD gene led to the isolation of another five genes: pelA, B, C, E, and F. These genes differ in their hybridization patterns with probes containing either the entire pelD gene, or 5' or 3' parts thereof. By partial sequencing, and expression in an A. niger transformant containing multiple copies of the pelA gene, we show that this gene, which hybridizes strongest with the pelD gene, encodes the other major pectin lyase from Ultrazym, PLII.
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PMID:Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): indications of a pectin lyase gene family in A. niger. 222 45

The study was to compare growth and enzymatic activity of Microsporum gypseum and Trichophyton ajelloi isolates from sewage sludge. Agar media and the API-ZYM test were used. The isolates showed weak gelatinase, catalase and urease activities and did not produce cellulase, pectate lyase and polygalacturonase. In some strains poor amylase and DNA-se activities were observed. No strain was able to hydrolyze casein. The strains were found to hydrolyze tributyrin, rapeseed oil and Biodiesel oil and to grow on Diesel oil medium. On the medium containing tributyrin and on the media with rapeseed oil and Biodiesel oil additions, inhibition and stimulation of fungal growth was observed, respectively. Diesel oil did not affect the growth of these fungi. The growth and enzymatic activity of M. gypseum was found to be better than the growth and activity of T. ajelloi. Higher enzymatic activity can be associated with the pathogenicity of M. gypseum.
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PMID:[A study of the growth and enzymatic activity of Microsporum gypseum and Trichophyton ajelloi isolates from sewage sludge]. 1792 96