Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ability of the two cultures Penicillium sp. and Fusarium sp. to synthesize extracellular pectolytic enzymes was investigated. The cultivation conditions providing a high level of the biosynthesis of these enzymes were identified. The methods of isolating the enzymes by means of organic solvents were developed. The pectinase from Penicillium sp. showed a higher thermostability whereas that from Fusarium sp. displayed a greater acid resistance. Using glutaraldehyde and titanium salts, active immobilized forms of pectinases on silica carriers were prepared.
 ...
PMID:[Pectolytic enzymes formed by Penicillium and Fusarium micromycetes]. 708 28

Extrusion technology has been used successfully to improve the nutritive value of full-fat oilseeds via shear force and heat applied during passage through the extruder, exposing more peptide bonds to enzymatic hydrolysis. However, the fibrous portion of the seeds is largely intact after extrusion. Therefore, application of carbohydrase mixtures targeting nonstarch polysaccharides might further improve the nutritive value of extruded full-fat oilseeds. This hypothesis was investigated in a study involving 6 ileal-cannulated barrows (average initial BW = 75.0 kg) fed extruded full-fat, soybean- (FFSB) based diets, without or with a carbohydrase mixture (CM) to determine apparent ileal digestibility (AID) of energy and standardized ileal digestibility (SID) of AA. The CM supplement provided 500, 50, 400, 1200, 450, and 45 units of pectinase, cellulase, mannanase, xylanase, glucanase, and galactanase, respectively, per kilogram of diet. A basal diet was formulated to contain FFSB as the sole source of AA, whereas the CM diet was formulated by supplementing the basal diet with CM. Diets contained titanium dioxide as an indigestible marker. Diets were fed in a 2-period crossover design to give 6 observations per diet. Each experimental period lasted 7 d, including a 5-d adaptation period and 2-d ileal digesta collection period. The SID of N and AA were calculated using published values for ileal endogenous N and AA losses from our laboratory. Enzyme supplementation increased (P < 0.01) AID values of DM (68.7 vs. 65.7%), GE (74.6 vs. 70.5%), and N (64.2 vs. 58.5%). Greater (P < 0.05) AID values were recorded for Leu, Met + Cys, Thr, Cys, Ser, and Tyr in the CM diet compared with the basal diet. Compared with the basal diet, the CM diet had greater SID values for N (73.7 vs. 68.6%; P < 0.01), Met + Cys (59.9 vs. 52.2%; P < 0.05), and Thr (66.3 vs. 61.2%; P < 0.05). The average SID for indispensable and total AA increased by 3.4% and 3.8% units, respectively, after enzyme supplementation. In conclusion, enzyme supplementation increased the nutritive value of extruded FFSB for finishing pigs.
 ...
PMID:Effect of a carbohydrase mixture on ileal amino acid digestibility in extruded full-fat soybeans fed to finishing pigs. 2266 49