Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Structural characterisation of an anti-ulcer polysaccharide (GL-BIII), purified from leaves of Panax ginseng C.A. Meyer, was studied. Methylation analysis indicated that GL-BIII consisted mainly of terminal Arap, 4- or 5-substituted Ara, 2,4-disubstituted Rha, 4- and 6-substituted Gal, and 3,6-disubstituted Gal. Single radial gel diffusion using beta-glucosyl-Yariv antigen indicated that GL-BIII contained a small proportion of a beta-(1-->3,6)-galactan moiety. GL-BIII also contained terminal, 4-substituted, and 3,4-disubstituted GalA, and terminal and 4-substituted GlcA. Base-catalysed beta-elimination suggested that some 2-substituted Rha in GL-BIII was attached to position 4 of a 4-substituted uronic acid. Both mild acid hydrolysis and endo-alpha-(1-->4)-polygalacturonase digestion of GL-BIII did not give fragments consisting mainly of GalA. Methylation analysis and GC-MS analysis of acidic oligosaccharides liberated by partial acid hydrolysis indicated that GL-BIII contained a GalA-(1-->4)-Rha unit in addition to longer acidic units consisting of 2-substituted Rha and 4-substituted GalA. Lithium-mediated degradation of GL-BIII followed by borohydride reduction gave small amounts of fractions containing long and intermediate neutral oligosaccharide-alditols and a large amount of a fraction containing short oligosaccharide-alditols. The long neutral oligosaccharide-alditol fraction mainly comprised 4- or 5-substituted Ara, terminal Galf, 6-substituted Glc, and 2-substituted Man, whereas the intermediate oligosaccharide-alditol fraction consisted mainly of terminal and 6-substituted Galp, 6-substituted Glc, and 2-substituted Man. Methylation analysis and GC-MS analysis of the short oligosaccharide-alditol fraction suggested that it contained at least 14 kinds of di- to tetra-saccharide-alditols such as Gal-(1-->2)-Rha-ol, Gal-(1-->4)-Rha-ol, Ara-->Ara-ol, and Ara-->Ara-->Ara-ol.
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PMID:Characterisation of an anti-ulcer pectic polysaccharide from leaves of Panax ginseng C.A. Meyer. 798 33

Unusual component sugars such as 2-methylfucose (2-Me-Fuc), 2-methylxylose (2-Me-Xyl), apiose (Api), and aceric acid (AceA) are contained in the bioactive pectins from Bupleurum falcatum, Glycyrrhiza uralensis, and Angelica acutiloba, but not in the other bioactive pectic heteroglycans and arabinogalactans from Chinese and Japanese herbs tested. Each pectin was digested with endo-alpha-(1-->4)-polygalacturonase, and gave two enzyme-resistant fractions, PG-1 (rhamnogalacturonan core with neutral sugar side-chains) and PG-2, and an oligogalacturonide fraction (PG-3) by gel filtration on Bio-gel P-30. The PG-2 fractions commonly consisted of unusual sugars such as 2-Me-Fuc, 2-Me-Xyl, Api, AceA, 3-deoxy-D-lyxo-heptulosaric acid (Dha), and 3-deoxy-D-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of each PG-2 gave a pentosyl-->6-deoxyhexosyl-->6-deoxyhexosyl-->pentitol fragment as a major oligosaccharide in addition to some neutral di- to trisaccharide alditols. Methylation analysis of the lithium degradation products from each PG-2 also indicated that these oligosaccharide alditols mainly consisted of terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, and 3'-linked Api. HPLC analysis showed that PG-2 had molecular heterogeneity. These results indicate that the bioactive pectins from medicinal herbs commonly consist of the minor KDO-containing region which resembles the rhamnogalacturonan II in plant cell walls.
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PMID:Existence of a rhamnogalacturonan II-like region in bioactive pectins from medicinal herbs. 799 76

The endo-alpha-(1-->4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1-->4)-Rha-ol (a major component), Ara-(1-->4)-Rha-ol, Glc-(1-->4)-Rha-ol, Ara-->Ara-->Ara-ol, and Ara-->Ara-->Ara-->Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-alpha-(1-->4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2') was purified. Component sugar analysis indicated that PG-2' contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-D-lyxo-heptulosaric acid (Dha), and 3-deoxy-D-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2' gave mainly a pentosyl-->6-deoxyhexosyl-->6-deoxyhexosyl-->pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosyl-->deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3'-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
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PMID:Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L. 814 69