Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.15 (pectinase)
2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Separate balance experiments were conducted to assess the potential of 2 commercial enzyme supplements to improve the nutritive value of dehulled lupin kernels. One supplement (enzyme A) contained primarily xylanase, pentosanase, hemicellulase activities and the other (enzyme B) primarily beta-glucanase, hemicellulase and pectinase activities. 2. The enzymes were added at 0, 0.25, 0.50, 0.75 and 1.00 g/kg in diets containing (g/kg) lupins 300, sorghum 543, casein 91, celite (as marker) 20, and vitamins and minerals 46. Control diets, with and without enzyme supplementation contained sorghum and casein at 800 and 134 g/kg, respectively, and no lupins. 3. Growth rates and food conversion ratios (FCR) of birds over 7 days were not affected by lupin inclusion or enzyme supplementation. FCR of broilers fed on the sorghum diet was improved by enzyme A but not by enzyme B. 4. Ileal starch digestibilities were slightly lower in birds fed on the lupin control diet (no enzyme) compared to the basal control diet. 5. Enzyme A increased the AME of the lupins from 10.01 MJ/kg DM to 11.65 MJ/kg DM when added at 0.5 g/kg. Higher rates of supplementation did not lead to further increases in AME values. 6. Enzyme A did not improve starch digestion in the diets but insoluble non-starch polysaccharides concentration in the digesta decreased (50.41-42.71 g/g acid insoluble ash marker) with increasing enzyme supplementation, suggesting that the improvement in AME was the result of increased fermentation of fibre in the hindgut. 7. Enzyme B did not affect the AME of lupins nor the ileal digestibility of nutrients, but caused an increase in the concentrations of soluble non-starch polysaccharides in the ileal digesta of chickens (19.21-35.77 mg/ml). This was accompanied by an increase in ileal digesta viscosity (11.4-34.2 m.Pa/s).
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PMID:Effects of enzyme supplementation on the nutritive value of dehulled lupins. 883 36

The effect of a new multicarbohydrase supplement of cell wall degrading activities on the nutritive value of corn, soybean meal (SBM), canola meal (CM), and peas for broiler chickens was investigated. Four isoenergetic and isonitrogenous corn (69% corn), SBM (30% SBM, 59% corn), CM (30% CM, 54% corn), and pea (30% peas, 52% corn) diets, without or with enzyme supplementation, were formulated to meet NRC specifications for broiler chickens (except for AME and CP, which were at 95 and 92% of NRC requirements, respectively). The enzyme supplement supplied 1,000 U of xylanase, 400 U of glucanase, 1,000 U of pectinase, 120 U of cellulase, 280 U of mannanase, and 180 U of galactanase per kilogram of diet. Each diet was fed in a mash form to 9 replicate pens of 5 broilers from 5 to 18 d. When compared with the control treatment, enzyme addition to the corn diet improved (P < 0.05) feed-to-gain ratio, whereas the performance of birds fed the other 3 diets was not affected. An increase (P < 0.05) in total tract nonstarch polysaccharides (NSP) digestibility, ileal starch digestibility, and AMEn was observed in birds fed the enzyme-supplemented corn diet. An improvement (P < 0.05) in total tract NSP digestibility, ileal protein digestibility, and AMEn content with enzyme supplementation was observed for the SBM diet. However, nutrient digestibilities and AMEn of CM and pea diets were not affected (P > 0.05) by enzyme addition even though the NSP digestibilities increased significantly (P < 0.05). A significant increase (P < 0.05) in water-soluble NSP and a decrease (P < 0.05) in water-insoluble NSP concentration of ileal digesta was noted for birds fed all 4 enzyme-supplemented diets. It would appear from this study that the nutrient utilization of corn-SBM diet by broilers could be enhanced by using an appropriate multicarbohydrase enzyme supplement. The nutrient encapsulating effect of cell wall polysaccharides in SBM, CM, and peas may not be the only factor responsible for incomplete nutrient utilization. The improvement in feed efficiency and starch availability in birds fed corn diet likely resulted from the cell wall degrading activity of the enzyme supplement.
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PMID:Nutritive values of corn, soybean meal, canola meal, and peas for broiler chickens as affected by a multicarbohydrase preparation of cell wall degrading enzymes. 1615 8