Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.15 (
pectinase
)
2,440
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Torenia fournieri is a special plant with embryo sac partly protruding through the micropyle of ovule, and its egg cell, two synergids and part of the central cell can be clearly observed using light microscope (Plate
I-1
, 2). The feature of embryo sac of T. fournieri makes it easy to isolate egg cell and zygote, especially for physiologically mature and vigorous egg cell to study its fertilization mechanism. Egg cells of T. fournieri were isolated from embryo sacs 2 d after anthesis using enzymatic digestion or mechanical dissection after mild osmotic shock (Plate I-3, 4). About 5% egg cells (2-3 from 50 ovules) could be mechanically dissected within 2 h. When 0.1% cellulase and 0.1%
pectinase
were added into the solution, about 18% egg cells (8-10 from 50 ovules) could be isolated within 2 h (Plate I-5). Although egg cells could be isolated by mechanical dissection, they can be used in in vitro fertilization to probe fertilization mechanism without deleterious effect of enzymatic action on the surface of egg cell. But it is easier to isolate egg cells by using enzymatic digestion, and more egg cells can be obtained for the research of molecular biology. Using enzymatic digestion method, the zygote of Torenia fournieri was also isolated from the pollinated ovules (Plate I-7-10). Basing on our successful isolation of mature sperm cells, the isolation of egg cells of T. fournieri will make in vitro fertilization possible for the first time in a dicot plant.
...
PMID:[Isolation of egg cells and zygotes from Torenia fournieri]. 1612 Oct 9
Fourteen isolates of
Fusarium
were isolated from wilt affected tomato samples collected from 10 different states of India. Characterization of the fungal cultures based on morphology and sequencing of ITS rDNA revealed that they belonged to
Fusarium oxysporum
f.sp.
lycopersici
(
Fol
). Pathogenicity assay on two susceptible tomato cultivars showed all the 14 isolates were pathogenic and categorized in high-, moderate- and low-virulent groups. Differential host assay on Bonny Best (no resistant gene), UC82-L (harboring
I-1
), Fla.MH1 (harboring
I-1
and
I-2
) and I3R-1 (harboring
I-1
,
I-2
and
I-3
) tomato genotypes and PCR amplification with race-specific primers indicated that all the
Fusarium
isolates infecting tomato in India were belonging to race 1. Molecular diversity analysis based on ISSR markers revealed the presence of 3 distinct groups of
Fol
isolates. Abundant diversity was observed among the
Fol
isolates in harboring the virulence-related genes (
endo-polygalacturonase
gene
pg1
and tomatinases) and toxin production (fumonisin). However, presence of
pg1
does not correlate with virulence and the isolates carrying tomatinase 4 (
tom-4
) in combination with other tomatinase genes were of virulent group. Detection of fumonisin gene in six isolates of
Fusarium
infecting tomato indicated their toxigenic nature.
...
PMID:Molecular characterization and race identification of
Fusarium oxysporum
f. sp.
lycopersici
infecting tomato in India. 3312 53
