Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.15 (
pectinase
)
2,440
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An alkali extract of cell walls of Gossypium hirsutum L. was sequentially digested by
endo-polygalacturonase
(
EC 3.2.1.15
), arabinofuranosidase AN1571.2 (EC 3.2.1.55), endo-arabinase (
EC 3.2.1.99
), and rhamnogalacturonan hydrolase AN9314.2 (
EC 3.2.1.15
). The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, size-exclusion, and anion exchange chromatography. Fractions from the anion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOFMS. A new oligosaccharide (RGS29), which contained a rhamnogalacturonan dimer backbone with two galactose and two arabinose residues in the side chains, was found. Its structure was identified by 1D and 2D NMR spectra as follows: [Formula: see text].
...
PMID:Isolation and structural characterization of a novel oligosaccharide from the rhamnogalacturonan of Gossypium hirsutum L. 1835 94
