Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.15 (pectinase)
 2,440 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Water-soluble and alkaline-soluble crude polysaccharides which were separated from the roots or leaves of Panax ginseng C. A. Meyer, were compared for their anti-ulcer activity. Of these four polysaccharide fractions, the water-soluble crude polysaccharide fraction (GL-2) from the leaves and the alkaline-soluble crude polysaccharide fraction (GRA-2) from the roots prevented HCl/ethanol-induced ulcerogenesis in mice potently. The most potent fraction, GL-2, was further fractionated into four polysaccharide fractions by precipitation with cethyltrimethylammonium bromide, and the weakly acidic polysaccharide fraction, GL-4, showed the most potent inhibition of gastric lesion formation. The activity of GL-4 decreased after treatment with periodate or digestion with endo-polygalacturonase, indicating that the carbohydrate moiety may contribute to the expression of the activity. GL-4 was further purified by anion-exchange chromatography and gel filtration, and the most active purified polysaccharide, GL-4IIb1III was obtained. GL-4IIb1III (average relative molecular mass, 16,000 d) had the nature of a pectic polysaccharide, and was composed mainly of galactose and galacturonic acid with small proportions of rhamnose, arabinose, mannose, glucose, and glucuronic acid. GL-4IIIb1III prevented HCl/ethanol-induced ulcerogenesis in mice dose dependently.
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PMID:Purification of an anti-ulcer polysaccharide from the leaves of Panax ginseng. 147 Jun 69

A water-soluble crude polysaccharide fraction (BR-1) prepared from the root of Bupleurum falcatum L. (Japanese name = Saiko) prevented HCl/ethanol induced ulcerogenesis in mice significantly. BR-1 was fractionated into four polysaccharide fractions (BR-2, BR-3, BR-4, and BR-5) by the addition of cetyltrimethylammonium bromide, and the strongly acidic polysaccharide fraction BR-2 showed the most potent inhibition of gastric lesion formation. When BR-2 was further fractionated by anion-exchange chromatography, the most potent anti-ulcer activity was observed in the pectin-like polysaccharide, bupleuran 2IIc. Bupleuran 2IIc was homogeneous as determined by electrophoresis and gel filtration. Bupleuran 2IIc was composed mainly of galacturonic acid with small proportions of arabinose, rhamnose, and galactose, and its average relative molecular mass was estimated to be 63,000 d. BR-2 lost most of its activity after treatment with periodate or digestion with endo-polygalacturonase indicating that the polygalacturonan region and/or the molecular mass may contribute to activity.
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PMID:Purification of anti-ulcer polysaccharides from the roots of Bupleurum falcatum. 181 48