Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.15 (
pectinase
)
2,440
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Synthesis of ethylene in static cultures as well as the effect of endogenous and exogenous ethylene on the synthesis of
polygalacturonase
by Aspergillus niger were determined. This strain produced maximum ethylene amounts when cultured at 30 degrees C for 3 d. The effect of adding ethylene precursors (citrate-cycle intermediates) on ethylene production was investigated.
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intracellular and extracellular
polygalacturonase
production was obtained with 2-oxoglutaric, pyruvic and fumaric acids, and with glutamic acid too. Addition of ethylene to the culture medium also increased the synthesis of
polygalacturonase
, although to a lower degree than when glutamic acid was added.
...
PMID:Regulation of the production of polygalacturonase by Aspergillus niger. 1242 19
Arabinogalactan-proteins (AGPs) are a class of highly glycosylated, hydroxyproline-rich glycoproteins that function in plant growth and development. Tomato LeAGP-1 represents a major AGP expressed in cultured cells and plants. Based on cDNA and amino acid sequence analyses along with carbohydrate and other biochemical analyses, tomato LeAGP-1 is hypothesized to be a classical AGP localized to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor. Here, this hypothesis was tested and supported with the following experiments. First, tomato (Lycopersicon esculentum, cv. UC82B) cotyledon protoplasts were isolated following cell wall digestion with cellulase and
pectinase
, and LeAGP-1 was immunolocalized to the plasma membrane with a LeAGP-1 antibody. Second, LeAGP-1 was shown to be a major AGP component in plasma membrane vesicles from tomato cv. Bonnie
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suspension-cultured cells by Western blot analysis with the LeAGP-1 antibody. Third, fluorescence microscopy of plasmolysed, transgenic tobacco (Nicotiana tabacum BY-2) suspension-cultured cells expressing a green fluorescent protein (GFP)-LeAGP-1 fusion product demonstrated localization to the plasma membrane and Hechtian threads. Fourth, the GFP-LeAGP-1 fusion protein was present in plasma membrane preparations from these transgenic tobacco cells by Western blot analysis with a GFP antibody. Fifth, GFP-LeAGP-1 secreted into the culture media contained ethanolamine, presumably attached to the C-terminal amino acid residue, consistent with its processing and release from the plasma membrane. Thus, these data support the hypothesis that LeAGP-1 is localized to the plasma membrane via a GPI anchor and suggest possible roles for LeAGP-1 in cellular signalling and matrix remodelling.
...
PMID:Tomato LeAGP-1 is a plasma membrane-bound, glycosylphosphatidylinositol-anchored arabinogalactan-protein. 1503 67
Fourteen isolates of
Fusarium
were isolated from wilt affected tomato samples collected from 10 different states of India. Characterization of the fungal cultures based on morphology and sequencing of ITS rDNA revealed that they belonged to
Fusarium oxysporum
f.sp.
lycopersici
(
Fol
). Pathogenicity assay on two susceptible tomato cultivars showed all the 14 isolates were pathogenic and categorized in high-, moderate- and low-virulent groups. Differential host assay on Bonny
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(no resistant gene), UC82-L (harboring
I-1
), Fla.MH1 (harboring
I-1
and
I-2
) and I3R-1 (harboring
I-1
,
I-2
and
I-3
) tomato genotypes and PCR amplification with race-specific primers indicated that all the
Fusarium
isolates infecting tomato in India were belonging to race 1. Molecular diversity analysis based on ISSR markers revealed the presence of 3 distinct groups of
Fol
isolates. Abundant diversity was observed among the
Fol
isolates in harboring the virulence-related genes (
endo-polygalacturonase
gene
pg1
and tomatinases) and toxin production (fumonisin). However, presence of
pg1
does not correlate with virulence and the isolates carrying tomatinase 4 (
tom-4
) in combination with other tomatinase genes were of virulent group. Detection of fumonisin gene in six isolates of
Fusarium
infecting tomato indicated their toxigenic nature.
...
PMID:Molecular characterization and race identification of
Fusarium oxysporum
f. sp.
lycopersici
infecting tomato in India. 3312 53
