Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.143 (
poly(ADP-ribose) glycohydrolase
)
208
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have developed a rapid, highly reproducible assay to determine
poly(ADP-ribose) glycohydrolase
activity which measures directly the appearance of the reaction product. We also analysed the majority of different techniques which are used to determine
poly(ADP-ribose) glycohydrolase
activity and found that the apparent activity can vary extensively depending on the method used. Thin-layer chromatography using
PEI
-F-cellulose was the only method which evaluated directly the specific release of ADP-ribose; by comparison with this method, the other procedures gave an over- or under-estimation of 2- to 10-fold of the enzymatic activity. A rapid method of affinity chromatography has also been developed to synthesize and purify in high yield poly(ADP-ribose) (35% conversion of 1 mM NAD to poly(ADP-ribose)).
...
PMID:Rapid assay of poly(ADP-ribose) glycohydrolase. 332 77
