Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.108 (
lactase
)
2,133
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A method for the histochemical demonstration of "hetero-beta-galactosidase" was elaborated. The enzyme is demonstrated in cryostat sections by the semipermeable membrane technique. Pairs of membranes--one pre-washed in saline--are used. The most sensitive method is post-coupling demonstration with 6-Br-2-naphthyl-beta-D-glucoside. The incubation time must be short, to avoid diffusion. The method allows cellular localization. The method with alpha-naphthyl-beta-D-glucoside and hexazonium-p-rosaniline is less sensitive, but localization is better. Indigogenic methods are the least sensitive. The enzyme is localized in the supranuclear zone of differentiated enterocytes of the human, monkey and rabbit small intestine, with maximum activity in the jejunum. The activity of the enzyme is low in patients with coeliac sprue, in the active phase of the disease. In isolated
lactase
deficiency it is normal. In the kidney, the enzyme is localized chiefly in the cytoplasm of the
proximal tubule
cells.
...
PMID:Demonstration of "hetero-beta-galactosidase" "in situ". 9 37
