Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.108 (lactase)
 2,133 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The expression of small intestinal hydrolases associated with the enterocyte brush border membrane was studied in human colon cancers and foetal colons, by means of monoclonal antibodies against human small intestinal sucrase-isomaltase (SI), maltase-glucoamylase (MGA), lactase (L), aminopeptidase N (APN), and dipeptidylpeptidase IV (DPP-IV). The enzymes were visualized by indirect immunofluorescence on cryostat sections of tumors developed in nude mice with 6 human colon carcinoma cell lines (HT-29, Caco-2, SW-480, HRT-18, HCT-8R, and Co-115), of 27 primary colorectal carcinomas from patients, and of human foetal (16 to 20 weeks of gestation) and normal adult small intestines and colons. All 5 monoclonals bound to the brush border of the adult small intestine, but not to that of the adult colon mucosa. Antibodies against SI, APN and DPP-IV also bound to the brush border of the foetal colons, to apical borders in HT-29 and Caco-2 tumors in nude mice, and to brush border-like structures in 7/27 tumors from patients. No binding was observed for MGA and L in either tumors or foetal colons. Binding of anti-SI antibodies to the brush border of the juxta-tumoral mucosal epithelium was observed in 9/11 samples tested. These data indicate that some colon tumors exhibit a typical pattern of enterocytic differentiation which is of foetal type and which involves at least 3 brush border membrane hydrolases. Monoclonal antibodies to small intestinal hydrolases may, therefore, be important tools for identification and characterization of some differentiated colonic tumors.
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PMID:Immunohistological evidence, obtained with monoclonal antibodies, of small intestinal brush border hydrolases in human colon cancers and foetal colons. 638 73

PURPOSE: To review the demographic characteristics of Asian and Pacific Islander Americans (APIAs) and their health care needs. METHODS: The author reviewed the 1990 Census data, later Current Population Surveys, monographs, books, and the medical literature on APIAs based on MEDLINE and other sources. FINDINGS: APIAs are the fastest growing minority in the U.S. They are mostly foreign­born, highly diversified, heterogeneous, bipolar in socioeconomic status, and concentrated in the West and metropolitan areas. APIAs have many health care needs: lack of health data, ethnocultural barriers, and high frequency of hepatitis B and tuberculosis and certain genetic disorders such as thalassemia and lactase deficiency. It is also questionable whether some U.S. norms and standards based on non­APIA subjects are appropriate for APIAs. CONCLUSIONS: APIAs are a fast growing minority whose many unmet health care needs have been overshadowed by the myth of a model minority. The health care system should address these needs and assure equal access to health services for all minorities. KEY WORDS: Asian Americans, Culture, Ethnicity, Health Services Accessibility, Health Education, Health Policy, Hepatitis B, Minority Groups, Thalassemia, Tuberculosis
Asian Am Pac Isl J Health 1993
PMID:Asian and Pacific Islander Americans: An Overview of Demographic Characteristics and Health Care Issues. 1156 40

The frequency of lactase persistence varies widely in human populations. Study showed that the T allele of a C/T transition 13910bp upstream from exon 1 of lactase gene (LCT) was completely associated with lactase persistence in a Finnish population. To evaluate if the frequency of -13910T allele was in concordance with the lactase persistence in northern Chinese populations, in this study, we used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to detect the lactase -13910T allelic frequency in 5 northern Chinese populations for the first time. Results showed that the T allele frequency was low in these populations and that it did not match the lactase persistence phenotype in these populations. Therefore the -13910T allelic frequency can not serve as a predictor of the lactase persistence in these populations and this suggests the existence of other possible mechanisms of lactose tolerance in Chinese populations.
Asia Pac J Clin Nutr 2007
PMID:The lactase gene -13910T allele can not predict the lactase-persistence phenotype in north China. 1804 17

The level of lactose reduction in milk necessary to alleviate the signs and symptoms of lactose intolerance has received little study. The purpose of this study was to determine whether 50% lactose-reduction in milk is adequate to alleviate the signs and symptoms of lactose maldigestion. even when large amounts of milk are consumed. Seven healthy subjects with proven lactose maldigestion consumed graded doses of whole cow's milk and 50% lactose reduced (LR) whole milk to determine the amount which could be consumed before breath hydrogen rose >20 ppm. This threshold was exceeded on average with 500 ml of 50% LR milk and 200 ml of whole milk. Whole milk produced significantly more breath hydrogen (P<0.05) and maldigestion symptoms (P<0.05) at all levels than the 50% LR milk. These results suggest that milk with as little as 50% lactose reduction can play a major role in the diet of individuals with lactase deficiency.
Asia Pac J Clin Nutr 1992 Dec
PMID:The effectiveness of 50% lactose-reduced milk in alleviating milk intolerance. 2432 41