Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.108 (lactase)
 2,133 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Unsubstituted naphthyl substrates were found to be superior to substituted naphthyl, indolyl and hydroxyquinoline substrates for the histochemical demonstration of alpha-mannosidase, alpha-galactosidase, hetero-beta-glycosidase, glucoamylase and sucraseisomaltase, equivalent for beta-N-acetylglucosaminidase and lactase-beta-glucosidase, and inferior for beta-glucuronidase and acid beta-galatosidase. Aldehyde fixation is necessary for the localization of lysosomal glycosidases with naphthyl substrates. 1-naphthyl substrates are suitable for the detection of acid glycosidases in lysosomes and hetero-beta-glysocidase in the cytoplasm of animal cells, and 2-naphthyl substrates can be employed for the demonstration of microvillous glycosidases and for the evaluation of the total activity of soluble glycosidases with semipermeable membranes. When naphthyl substrates are used coupling should be carried out simultaneously and hexazotized pararosaniline is the coupling reagent of choice.
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PMID:Localization of glycoidases with naphthyl substrates. 5 19

Activities of the brush border enzymes alkaline phosphates, leucine aminopeptidase and lactase and the lysosomal enzymes alpha-mannosidase and beta-N-acetylglucosaminidase increased in the serum of newly-born lambs fed colostrum. Feeding lipid and protein components of colostrum and bovine serum resulted in enzyme responses similar to those observed after feeding colostrum. Activities of each of the enzymes increased in mesenteric lymph collected from newly-born lambs when immunoglobulins were being absorbed from the jejunum and ileum.
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PMID:Intestinal brush border and lysosomal enzymes and immunoglobulin absorption in the newly-born lamb. 41 Apr