Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
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Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:3.1.6.4 (
chondroitinase
)
2,039
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Oncomodulin
, an ~12 kDa Ca(2+)-binding protein secreted from activated macrophages, has been shown to promote axonal regeneration from retinal ganglion cells (RGCs) following optic nerve injury. However, to date, the axonal growth-promoting capacity of
oncomodulin
in other models of 'regenerative failure' has not been evaluated. We assessed the capability of preconditioning treatment with
oncomodulin
to promote sensory axonal regeneration in an in vitro spot model of regenerative failure, and across the dorsal root zone (DREZ) after root crush injury. Neither the direct exposure of adult rat DRGs to
oncomodulin
, nor preconditioning of DRGs by intraganglionic injection of
oncomodulin
, stimulated axonal outgrowth in the in vitro proteoglycan spot gradient assay. However, direct exposure of unconditioned DRGs to both
oncomodulin
and db-cAMP in vitro, as well as preconditioning of DRGs with the combined treatment in vivo, resulted in significant, albeit modest, neurite extension across the inhibitory proteoglycan barrier. We next quantified axon regeneration through the C8 DREZ in adult rats after
oncomodulin
and/or db-cAMP preconditioning and
chondroitinase
(ChABC) injection into the DREZ immediately following a root crush injury. Axonal regeneration across the DREZ was not observed in control animals, or after injection of ChABC-alone. Treatment with
oncomodulin
- or db-cAMP-alone resulted in extremely sparse regeneration. However, significant, but meager, sensory axon regeneration across the DREZ was observed using the
oncomodulin
/ db-cAMP combination (p<0.001), supporting findings from previous studies suggesting that cAMP is necessary for the growth-promoting effects of
oncomodulin
. Although our results support a role for
oncomodulin
in macrophage-induced axonal regeneration, the effects of
oncomodulin
/db-cAMP on sensory regeneration were extremely limited in comparison to previous studies in the same injury model using zymosan.
...
PMID:Oncomodulin affords limited regeneration to injured sensory axons in vitro and in vivo. 2207 58
