Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.6.4 (chondroitinase)
 2,039 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Production and characterization of hemidesmosome-specific mouse monoclonal antibodies 3A1 and 8A12 raised against the oral squamous carcinoma line cells were previously reported. In this study, further investigations were carried out to characterize these antigens using those cells. Immunoaffinity-purified 3A1 antigen was identified as a 180kD glycoprotein [205kD under reduced conditions]. Acetate membrane electrophoresis of disaccharides originating from this antigen digested by chondroitinase ABC showed that this protein was associated with hyaluronate. Immunoprecipitates by 3A1 and 8A12 antibodies were composed of two bands: 180kD and 140kD [205kD and 125kD by reduction], and were similar to integrin alpha 6 beta 4 precipitated by monoclonal anti-alpha 6 subunit antibody. Immunoprecipitation-Western Blot analysis showed that the 140kD [125kD] protein precipitated by 3A1 and 8A12 was alpha 6 subunit and that the 180kD [205kD] beta 4 subunit. After the alpha 6 subunit was immunodepleted, only the 180kD [205kD] band was immunoprecipitated by 3A1 or 8A12. Both 3A1 and 8A12 blocked the adhesion of LMF5 cells to laminin. These data indicated that 3A1 and 8A12 recognized integrin beta 4 subunit and that integrin alpha 6 beta 4 functioned as a laminin receptor on these cells. It was suggested that hyaluronan detected in the 3A1 antigen was associated with two putative hyaluronan-binding motifs in the extracellular domain of the integrin beta 4 subunit.
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PMID:[Investigations on cell surface molecules expressed by human oral squamous carcinoma line cells HSC-3, LMF 4 and LMF 5, with metastatic potential]. 789 68

Hepatocyte growth factor (HGF) promotes the growth not only of hepatocytes but also of several other types of cells such as cytotrophoblasts and endothelial cells. Recent studies have revealed that HGF is trapped in the extracellular (ECM) matrix through heparan sulphate in vivo, thereby acting as a mitogen for hepatocytes in cooperation with heparan sulphate. In this study, we detected HGF protein in chorionic tissue and placental tissue extracts, and found that HGF and heparan sulphate were co-distributed in the endothelial basement membrane and trophoblast basement membrane on immunohistochemical examination. The rates of DNA synthesis in primary cultured cytotrophoblasts and human umbilical vein endothelial cells (HUVEC) cultured on HGF-bound Matrigeltrade mark were 6-8 times those of control cytotrophoblasts and HUVEC. When Matrigeltrade mark dishes were pretreated with heparinase and heparitinase prior to binding of HGF, stimulation of DNA synthesis was markedly decreased. A considerable decrease in stimulation of DNA synthesis was observed following washing of HGF-bound Matrigeltrade mark with 1 m acetic acid, 1 m NaCl and 0.1 per cent trypsin, but not following treatment with chondroitinase ABC. These observations suggest that HGF can be trapped in ECM in vivo, thereby acting as a mitogen for cytotrophoblasts and placental vein endothelial cells in cooperation with heparan sulphate.
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PMID:Stimulation of DNA synthesis in trophoblasts and human umbilical vein endothelial cells by hepatocyte growth factor bound to extracellular matrix. 1052 23