Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.6.4 (chondroitinase)
 2,039 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monoclonal anti-dermatan sulphate antibodies were produced with hybridization after immunizing BALB/c mice with dermatan sulphate (DS). These antibodies were tested for cross-reactivity to double-stranded DNA (dsDNA) and heat-denatured DNA (ssDNA). Five clones had binding activity to DS, dsDNA and ssDNA in enzyme-linked immunosorbent assay, but no clones binding to DS alone were produced. Two (2H8 and 3C4) of the five clones were selected for analysis. Their binding activity to DS, ssDNA and dsDNA were inhibited by DS, dsDNA and ssDNA. DNase I treatment abolished the binding activity to dsDNA and ssDNA, completely, but had no effect on the binding activity to DS. On the other hand, chondroitinase ABC treatment of the solid-phased DS augmented the binding to DS. The inhibition assay using digested fractions suggested that the epitope recognized by 2H8 and 3C4 clones could not be in delta Di-4S or delta Di-diSB but in the linkage regions of DS.
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PMID:Generation of monoclonal anti-dermatan sulphate antibodies cross-reacting to calf thymus DNA. 270 70

Monoclonal anti-dermatan sulfate antibodies were produced by immunizing BALB/c mouse with dermatan sulfate (DS) derived from porcine skin. These antibodies were tested for cross-reactivity to double stranded calf thymus DNA(ds.DNA) and heat-denatured DNA (ss.DNA). Five clones (including 2H8 and 3C4 clones) had binding activities to DS, ds.DNA and ss.DNA in solid phase enzyme linked immunosorbent assay, but no clones binding to DS alone were produced. 2H8 and 3C4 clones could activate mouse complement when they bound to the DS and ss.DNA. The abilities of DS, ds.DNA and ss.DNA to inhibit binding of 2H8 and 3C4 clones to solid phased DS, ds.DNA and ss.DNA were examined. Their binding activities were inhibited by any of DS, ds.DNA and ss.DNA. DNase I treatment abolished the binding activities of 2H8 and 3C4 clones to ds.DNA and ss.DNA completely, but had no effect on the binding activities to Ds. On the other hand, chondroitinase ABC treatment of DS augmented the binding activities of 2H8 and 3C4 to DS. DS digested completely with chondroitinase ABC inhibited the binding activities to DS of 2H8 and 3C4 clones, but not any of delta Di-4S and delta Di-diSB produced from DS digested with this enzyme inhibited the binding activities. Therefore, it was suggested that the epitope recognized by 2H8 and 3C4 clones could not be in delta Di-4S or delta Di-diSB but in the linkage regions of DS.
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PMID:[Generation of monoclonal anti-dermatan sulfate antibodies cross-reacting to calf thymus DNA. Analysis of "anti-DNA antibody" directly binding to dermatan sulfate]. 278 51