Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.6.4 (chondroitinase)
 2,039 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Thirty-nine strains of gram-positive microaerophilic cocci isolated from cases of heifer and dry-cow mastitis were biochemically characterized with the API 50E and API-ZYM test kit systems, gas-liquid chromatography for analysis of end products of glucose metabolism, and anaerobic biochemical tests (L. V. Holdeman, E. P. Cato, and W. E. C. Moore, Anaerobe Laboratory Manual, Virginia Polytechnic Institute, Blacksburg, 1977). Strains were screened for production of a variety of extracellular enzymes on substrate-containing agar plates and for hemolysin and coagulase production. Antibiotic susceptibility and sensitivity tests were also performed. The microaerophilic cocci displayed homogeneity with respect to the majority of the biochemical tests used; i.e., greater than or equal to 90% of the strains were consistently positive or negative in any one test and probably represent one species. All produced deoxyribonuclease, ribonuclease, and hyaluronidase, and 92% were positive for chondroitin sulfatase. Catalase and coagulase tests were negative. Greening was observed on bovine blood agar. Acetic and succinic acids were produced by all strains as the only detectable products of glucose metabolism. The strains were susceptible to penicillin G, cefoxitin, doxycycline, and chloramphenicol and were resistant to clindamycin, novobiocin, and metronidazole. Their taxonomic position remains unclear.
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PMID:Biochemical characterization of unidentified microaerophilic cocci isolated from heifer and dry-cow mastitis. 39 19

Using the reagents from the CEA-Roche kit, we found that solutions containing only glycosaminoglycans (GAG) yielded false concentrations of carcinoembryonic antigen (CEA), and mixtures of CEA and GAG produced falsely high values. On the other hand, solutions of GAG yielded no additional CEA concentration when reagents from the Abbott CEA kit were used; rather, the CEA result was decreased with the Abbott kit for a CEA solution also containing GAG. These effects of GAG were not ascribable to contamination, because neither gel filtration nor ion-exchange column chromatography separated the false Roche CEA content related to GAG from their peaks of uronic acid or from their anticoagulant activity. In addition, an enzyme specific for GAG eliminated these GAG-derived false concentrations. Both the positive and negative effects are correlated with concentrations of GAG. We found that the concentration of GAG could be decreased in a solution containing plasma proteins by either heating (70 degrees C, 15 min) or treating with perchloric acid (0.6 mol/L). The former is superior because essentially all GAG added to a solution containing plasma proteins were removed by heat, whereas as much as 25% to 80% of the GAG still remained after acid treatment. The effect of GAG was also completely eliminated by treating the specimens with chondroitinase ABC lyase (EC 4.2.2.4).
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PMID:Interference with carcinoembryonic antigen radioimmunoassays by glycosaminoglycans, and their removal. 631 62