Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.6.12 (
chondroitinase
)
2,183
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Factor J (FJ) is a complement inhibitor that acts on the classical and the alternative pathways. We demonstrated FJ-cell interactions in fluid phase by flow cytometry experiments using the cell lines Jurkat, K562, JY, and peripheral blood lymphocytes. FJ bound to plastic plates was able to induce in vitro adhesion of these cells with potency equivalent to fibronectin. As evidence for the specificity of this reaction, the adhesion was blocked by MAJ2, an anti-FJ monoclonal antibody, and by soluble FJ. Attachment of the cells required active metabolism and cytoskeletal integrity. The glycosaminoglycans heparin, heparan sulfate, or chondroitin sulfates A, B, and C inhibited to varying degrees the binding of FJ to cells, as did treatment with
chondroitinase
ABC. In the search for a putative receptor, a protein of 110 kDa was isolated by affinity chromatography, and microsequence analysis identified this protein as
nucleolin
. Confocal microscopy evidenced the presence of
nucleolin
in cell membrane by immunofluorescence with monoclonal (D3) and polyclonal anti-
nucleolin
antibodies in Jurkat cells. The interaction FJ-
nucleolin
was evidenced by Western blot and enzyme-linked immunosorbent assay. Furthermore, purified
nucleolin
and D3 inhibited adhesion of Jurkat cells to immobilized FJ, suggesting that the interaction was specific and that
nucleolin
mediated the binding.
...
PMID:Cellular adhesion mediated by factor J, a complement inhibitor. Evidence for nucleolin involvement. 982 33
