Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.6.1 (sulfatase)
 3,205 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mammary tissue from 44 primiparous mice at various stages of pregnancy, lactation, and involution were stained for aryl sulfatase (lysosomal marker enzyme) activity and prepared for electron microscopy. Stereological techniques were used to determine the distribution of lysosomes per unit of cytoplasmic area in mammary epithelial cells. The number of primary lysosomes (containing only enzyme) was stable throughout the study, except for a temporary increase following parturition. Secondary lysosomes (containing substrate undergoing active digestion) designated as either telolysosomes or dense bodies were more frequent in mammary epithelial cells from animals in the late involution and early pregnancy stages than in lactating animals. However, telolysosomes did increase temporarily at the onset of lactation and casein micelles were identified within secondary lysosomes throughout the lactation stage. The frequencies for endoplasmic reticulum and Golgi apparatus containing aryl sulfatase reaction product were highest in secretory cells, when these structures were predominant. Shifts in lysosomal populations throughout the study suggests that lysosomes may have an active role within the mammary epithelial cells during differentiation and secretion as well as during involution.
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PMID:Distribution of the lysosomal enzyme aryl sulfatase in murine mammary tissue through pregnancy, lactation, and involution. 225 80

A sensitive and robust method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for quantitation of 13 phytoestrogens and related metabolites in rat serum samples. A new type of column, the Kinetex core-shell C18 column, was applied for rapid separation of the target analytes in 10min. Two enzymes, sulfatase H-1 and gulcuronidase H-5 from Helix pomatia were compared on the efficiency of releasing the conjugated forms of the target analytes to their free forms in serum samples. The method detection limit (MDL) defined as three times the signal to noise ratio in spiked serum matrix-based solutions was in the range of 0.1-3.5ng/mL. The linear dynamic calibration was in the broad range of 0.2-500ng/mL for all target compounds. Thirty-two rat serum samples from the rats that were fed with diets containing either casein or soy protein isolates with various amounts of isoflavones for 8 weeks were analyzed for the target analytes with the developed method. Nine target analytes were detected in the serum samples. Those detectable compounds are all the metabolites of the dietary isoflavones, suggesting that the diet isoflavones were mostly metabolized to their metabolites in rat.
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PMID:A robust analytical method for measurement of phytoestrogens and related metabolites in serum with liquid chromatography tandem mass spectrometry. 2681 20