Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.4.37 (
CNPase
)
539
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An improved method for the assay of cyclic nucleotide phosphodiesterases (CNPases) is described in which residual 3H-cAMP or 3H-cGMP is separated from products by chromatography on thin layers of
PEI
-cellulose. Comparison of
CNPase
activity in crude or purified kidney enzyme assayed by the
PEI
-cellulose method with a batch anion-exchange resin method showed serious underestimation of activity by the latter method. The batch method underestimated
CNPase
activity by 34-82%. The error was due to substantial binding of nucleosides (adenosine and guanosine) to the resin, which is assumed not to occur by those using this method.
...
PMID:Comparison of batch and chromatographic assays of cyclic nucleiotide phosphodiesterases. 18 7
