Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.4.3 (phospholipase C)
 18,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
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PMID:A transient receptor potential channel expressed in taste receptor cells. 1236 8

A specific laryngeal sensory epithelium (SLSE), which includes arrays of solitary chemoreceptor cells, is described in the supraglottic region of the rat. Two plates of SLSE were found, one on each side of the larynx. The first plate was located in the ventrolateral wall of the larynx, and the second was located in the interarytenoidal region. In SLSE, immunoblotting showed the presence of alpha-gustducin and phospholipase C beta2 (PLCbeta2), which are two markers of chemoreceptor cells. At immunocytochemistry, laryngeal immunoreactivity for alpha-gustducin was localized mainly in solitary chemosensory cells. Double-label immunocytochemistry using confocal microscopy demonstrated that alpha-gustducin-expressing cells in large part colocalize type III IP3 receptor (IP3R3), another key molecule in bitter taste perception. However, some IP3R3-expressing cells do not colocalize alpha-gustducin. At ultrastructural immunocytochemistry, these cells showed packed apical microvilli, clear cytoplasmic vesicles, and cytoneural junctions. SLSE was characterized by high permeability to a tracer due to poorly developed junctional contacts between superficial cells. Junctions were short in length and showed little contact with the terminal web. Ultrastructural analysis showed deep pits among the superficial cells. In SLSE, high density of intraepithelial nerve fibers was found. The lamina propria of the SLSE appeared thicker than that in other supraglottic regions. It was characterized by the presence of a well-developed subepithelial nerve plexus. The immunocytochemical and ultrastructural data suggested that SLSE is a chemoreceptor located in an optimal position for detecting substances entering the larynx from the pharynx or the trachea.
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PMID:Identification and characterization of a specific sensory epithelium in the rat larynx. 1521 60

The mammalian signal transduction apparatus utilized by vomeronasal sensory neurons (VSNs) in the vomeronasal organ (VNO) has been richly explored, while that of reptiles, and in particular, the stinkpot or musk turtle Sternotherus odoratus, is less understood. Given that the turtle's well-known reproductive and mating behaviors are governed by chemical communication, 247 patch-clamp recordings were made from male and female S. odoratus VSNs to study the chemosignal-activated properties as well as the second-messenger system underlying the receptor potential. Of the total neurons tested, 88 (35%) were responsive to at least one of five complex natural chemicals, some of which demonstrated a degree of sexual dimorphism in response selectivity. Most notably, male VSNs responded to male urine with solely outward currents. Ruthenium Red, an IP3 receptor (IP3R) antagonist, failed to block chemosignal-activated currents, while the phospholipase C (PLC) inhibitor, U73122, abolished the chemosignal-activated current within 2 min, implicating the PLC system in the generation of a receptor potential in the VNO of musk turtles. Dialysis of several second messengers or their analogues failed to elicit currents in the whole-cell patch-clamp configuration, negating a direct gating of the transduction channel by cyclic adenosine monophosphate (cAMP), inositol 1,4,5-trisphosphate (IP3), arachidonic acid (AA), or diacylglycerol (DAG). Reversal potential analysis of chemosignal-evoked currents demonstrated that inward currents reversed at -5.7+/-7.8 mV (mean +/- s.e.m.; N=10), while outward currents reversed at -28.2+/-2.4 mV (N=30). Measurements of conductance changes associated with outward currents indicated that the outward current represents a reduction of a steady state inward current by the closure of an ion channel when the VSN is exposed to a chemical stimulus such as male urine. Chemosignal-activated currents were significantly reduced when a peptide mimicking a domain on canonical transient receptor potential 2 (TRPC2), to which type 3 IP3 receptor (IP3R3) binds, was included in the recording pipette. Collectively these data suggest that there are multiple transduction cascades operational in the VSNs of S. odoratus, one of which may be mediated by a non-selective cation conductance that is not gated by IP3 but may be modulated by the interaction of its receptor with the TRPC2 channel.
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PMID:Vomeronasal sensory neurons from Sternotherus odoratus (stinkpot/musk turtle) respond to chemosignals via the phospholipase C system. 1665 57

Inositol 1,4,5-trisphosphate receptor (IP3R) is one of the important calcium channels expressed in the endoplasmic reticulum and has been shown to play crucial roles in various physiological phenomena. Type 3 IP3R is expressed in taste cells, but the physiological relevance of this receptor in taste perception in vivo is still unknown. Here, we show that mice lacking IP3R3 show abnormal behavioral and electrophysiological responses to sweet, umami, and bitter substances that trigger G-protein-coupled receptor activation. In contrast, responses to salty and acid tastes are largely normal in the mutant mice. We conclude that IP3R3 is a principal mediator of sweet, bitter, and umami taste perception and would be a missing molecule linking phospholipase C beta2 to TRPM5 activation.
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PMID:Abnormal taste perception in mice lacking the type 3 inositol 1,4,5-trisphosphate receptor. 1792 4