Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.4.3 (
phospholipase C
)
18,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ehrlichia canis, etiologic agent of Canine Monocytic Ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. In this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as
phospholipase C
(
PLC
), protein kinase (PTK) and calcium channels as well as the role of iron in the E. canis proliferation in DH82 cells. Different inhibitory compounds were used for each component: Cytochalasin D (inhibits actin polymerization), Nocodazole (inhibits microtubule polymerization),
Neomycin
(
PLC
inhibitor), Genistein (PTK inhibitor), Verapamil (calcium channel blocker) and Deferoxamine (iron chelator). We observed a significant decrease in the total number of bacteria in infected cells treated suggesting that these cellular components analized are essentials to E. canis proliferation.
...
PMID:The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation. 2241 81
To investigate the mechanisms of
phospholipase C
(
PLC
)-mediated calcium (Ca
2+
) signaling in
Alternaria alternata
, the regulatory roles of
PLC
were elucidated using neomycin, a specific inhibitor of
PLC
activity. Three isotypes of
PLC
designated
AaPLC1
,
AaPLC2
, and
AaPLC3
were identified in
A. alternata
through genome sequencing. qRT-PCR analysis showed that fruit wax extracts significantly upregulated the expression of all three
PLC
genes
in vitro
. Pharmacological experiments showed that neomycin treatment led to a dose-dependent reduction in spore germination and appressorium formation in
A. alternata
. Appressorium formation was stimulated on hydrophobic and pear wax-coated surfaces but was significantly inhibited by neomycin treatment. The appressorium formation rates of neomycin treated
A. alternata
on hydrophobic and wax-coated surfaces decreased by 86.6 and 47.4%, respectively. After 4 h of treatment, exogenous CaCl
2
could partially reverse the effects of neomycin treatment.
Neomycin
also affected mycotoxin production in alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), and tentoxin (TEN), with exogenous Ca
2+
partially reversing these effects. These results suggest that
PLC
is required for the growth, infection structure differentiation, and secondary metabolism of
A. alternata
in response to physiochemical signals on the pear fruit surface.
...
PMID:Phospholipase C From
Alternaria alternata
Is Induced by Physiochemical Cues on the Pear Fruit Surface That Dictate Infection Structure Differentiation and Pathogenicity. 3269 73
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