Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:3.1.4.3 (
phospholipase C
)
18,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The intracellular location of various enzymes involved in the metabolism of phospholipids of Candida albicans was studied. Among the biosynthetic enzymes, phosphatidylserine synthetase was found to be localized in the microsomes; choline kinase and ethanolamine kinase were cytosolic; acyltransferase was localized in the particulate fraction and
glycerol kinase
and phosphatidic acid phosphatase were distributed in both the microsomal and cytosolic fractions. Phospholipase A and
phospholipase C
were abundant in the microsomes and
phospholipase C
was also detected in the cytosol. Lysophospholipase and glycerophosphocholine diesterase were distributed mainly in the mitochondria. Lipase activity was also detected in this fungus. Based on the enzymes detected in this study we have postulated pathways of phospholipid metabolism in C. albicans.
...
PMID:Subcellular localization of enzymes of phospholipid metabolism in Candida albicans. 228 83
We describe methods for automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid. Phospholipase C (
EC 3.1.4.3
) and sphingomyelin phosphodiesterase (EC 3.1.4.12) are reacted with lecithin and sphingomyelin, respectively, to liberate phosphocholine. Phosphocholine is then reacted with alkaline phosphatase, choline oxidase, peroxidase, and 4-aminoantipyrine to form a colored complex, for which the absorbance at 500 nm is measured with a centrifugal analyzer. Phosphatidylglycerol is hydrolyzed by phospholipase D (EC 3.1.4.4) to form glycerol, which is subsequently reacted with ATP and NAD+ in the presence of
glycerol kinase
and glycerol-3-phosphate dehydrogenase to yield NADH. The absorbance of the NADH formed is measured at 340 nm. These methods provide a simple, rapid, and accurate alternative to thin-layer chromatography for determination of phospholipids in amniotic fluid for assessment of fetal lung maturity.
...
PMID:Automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid. 380 1
