Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.4.3 (phospholipase C)
 18,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A macromolecular complex including an inactive and protease-sensitive form of levansucrase was isolated from phenol treatment of Bacillus subtilis. This complex has a weak affinity for hydroxyapatite. Its molecular weight is evaluated at 300 000. This levansucrase form may be activated either by Triton X-100, above its critical micellar concentration, or by phospholipase C. The enzyme form associated with the complex is quite different from the exocellular levansucrase, which is protease-insensitive and detergent-insensitive, shows high affinity for hydroxyapatite and has a molecular weight of 54 000. Triton X-100 disaggregates this complex. The active levansucrase released becomes protease-insensitive and has the same apparent sedimentation coefficient as the exocellular enzyme. Possible relationships between the existence of this trapped complex and the secretion process of levansucrase are discussed.
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PMID:Levansucrase of Bacillus subtilis. Characterization of a form isolated from phenol-treated cells and activated by Triton X-100. 676 58