Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.4.3 (
phospholipase C
)
18,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Intestinal passive Mg(2+) absorption, which is vital for normal Mg(2+) homeostasis, has been shown to be regulated by luminal proton. We aimed to study the regulatory role of intestinal acid sensors in paracellular passive Mg(2+) transport.
Omeprazole
enhanced the expressions of acid-sensing ion channel 1a (ASIC1a), ovarian cancer G protein-coupled receptor 1 (OGR1), and transient receptor potential vanilloid 4 in Caco-2 cells. It also inhibited passive Mg(2+) transport across Caco-2 monolayers. The expression and activation of OGR1 resulted in the stimulation of passive Mg(2+) transport via
phospholipase C
- and protein kinase C-dependent pathways. ASIC1a activation, on the other hand, enhanced apical HCO3 (-) secretion that led, at least in part, by a Ca(2+)-dependent pathway to an inhibition of paracellular Mg(2+) absorption. Our results provided supporting evidence for the roles of OGR1 and ASIC1a in the regulation of intestinal passive Mg(2+) absorption.
...
PMID:The roles of acid-sensing ion channel 1a and ovarian cancer G protein-coupled receptor 1 on passive Mg2+ transport across intestinal epithelium-like Caco-2 monolayers. 2437 28
The mechanism of proton pump inhibitors (PPIs) suppressing intestinal Mg
2+
uptake is unknown. The present study aimed to investigate the role of purinergic P2Y receptors in the regulation of Mg
2+
absorption in normal and omeprazole-treated intestinal epithelium-like Caco-2 monolayers.
Omeprazole
suppressed Mg
2+
transport across Caco-2 monolayers. An agonist of the P2Y
2
receptor, but not the P2Y
4
or P2Y
6
receptor, suppressed Mg
2+
transport across control and omeprazole-treated monolayers.
Omeprazole
enhanced P2Y
2
receptor expression in Caco-2 cells. Forskolin and P2Y
2
receptor agonist markedly enhanced apical HCO
3
-
secretion by control and omeprazole-treated monolayers. The P2Y
2
receptor agonist suppressed Mg
2+
transport and stimulated apical HCO
3
-
secretion through the G
q
-protein coupled-
phospholipase C
(
PLC
) dependent pathway. Antagonists of cystic fibrosis transmembrane conductance regulator (CFTR) and Na
+
-HCO
3
-
cotransporter-1 (NBCe1) could nullify the inhibitory effect of P2Y
2
receptor agonist on Mg
2+
transport across control and omeprazole-treated Caco-2 monolayers. Our results propose an inhibitory role of P2Y
2
on intestinal Mg
2+
absorption.
...
PMID:The inhibitory role of purinergic P2Y receptor on Mg
2+
transport across intestinal epithelium-like Caco-2 monolayer. 3003 68
