Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.4.3 (phospholipase C)
 18,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Recent studies have implicated various toxigenic bacteria and their toxins in the aetiology of sudden infant death syndrome (SIDS). Therefore the effect of six bacterial toxins on the cardiorespiratory system of the rabbit was studied as a model for SIDS. The toxins' effect on the heart rate, arterial blood pressure, and breathing of anaesthetized rabbits was determined and their action compared to that of endotoxin. Intravenous injection of Clostridium perfringens enterotoxin and alpha-toxin, Staphylococcus enterotoxin B, Escherichia coli heat-stable toxin (STa), Clostridium difficile toxin A and B reduced heart rate, blood pressure, respiration and increased, slowed and prolonged thorax expansion, and at higher concentrations caused sudden death without visible stress or trauma. A combination of a low concentration of enterotoxins caused a greater reduction of these activities and sudden death. These effects were generally similar to those produced by endotoxin. In non-anaesthetized rabbits, the toxins slowed metabolism until death occurred without agitation, spasms, visible distress or prolonged illness. Intestinal production of these toxins by toxigenic strains, when conditions are suitable, and their systemic absorption, could therefore cause SIDS by an endotoxin-like shock mechanism.
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PMID:Is cardiorespiratory failure induced by bacterial toxins the cause of sudden infant death syndrome? Studies with an animal model (the rabbit). 766 Mar 68

Hollow fiber cell fishing, based on HepG-2, SKOV-3, and ACHN cancer cells, and hollow fiber liquid/solid microextraction with HPLC were developed and introduced for researching the anticancer activity of Rhizoma Curcumae Longae, Radix Curcumae, and Rhizoma Curcumae. The structures of curcumin, demethoxycurcumin, and bisdemethoxycurcumin screened were identified and their contents were determined. The compound target fishing factors and cell apoptosis rates under the effect of the three medicines were determined. The binding sites (cell membrane and cell organelle) and binding target (phospholipase C) on the cell were researched. Hollow fiber liquid/solid-phase microextraction mechanism was analyzed and expounded. Before the application, cell seeding time, growth state and survival rate, compound nonspecific binding, positive and negative controls, repeatability in hollow fiber cell fishing with high-performance liquid chromatography; extraction solvent, sample pH, salt concentration, agitation speed, extraction time, temperature and sample volume in hollow fiber liquid/solid-phase microextraction with high-performance liquid chromatography were investigated. The results demonstrated that the proposed strategy is a simple and quick method to identify bioactive compounds at the cellular level as well as determine their contents (particularly trace levels of the bioactive compounds), analyze multicompound and multitarget entirety effects, and elucidate the efficacious material base in traditional medicine.
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PMID:Screening and quantification of anticancer compounds in traditional Chinese medicine by hollow fiber cell fishing and hollow fiber liquid/solid-phase microextraction. 2698