Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.4.3 (
phospholipase C
)
18,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mechanism of proton pump inhibitors (PPIs) suppressing intestinal Mg
2+
uptake is unknown. The present study aimed to investigate the role of purinergic P2Y receptors in the regulation of Mg
2+
absorption in normal and omeprazole-treated intestinal epithelium-like Caco-2 monolayers. Omeprazole suppressed Mg
2+
transport across Caco-2 monolayers. An agonist of the P2Y
2
receptor, but not the P2Y
4
or P2Y
6
receptor, suppressed Mg
2+
transport across control and omeprazole-treated monolayers. Omeprazole enhanced P2Y
2
receptor expression in Caco-2 cells. Forskolin and P2Y
2
receptor agonist markedly enhanced apical HCO
3
-
secretion by control and omeprazole-treated monolayers. The P2Y
2
receptor agonist suppressed Mg
2+
transport and stimulated apical HCO
3
-
secretion through the G
q
-protein coupled-
phospholipase C
(
PLC
) dependent pathway. Antagonists of
cystic fibrosis transmembrane conductance regulator
(
CFTR
) and Na
+
-HCO
3
-
cotransporter-1 (NBCe1) could nullify the inhibitory effect of P2Y
2
receptor agonist on Mg
2+
transport across control and omeprazole-treated Caco-2 monolayers. Our results propose an inhibitory role of P2Y
2
on intestinal Mg
2+
absorption.
...
PMID:The inhibitory role of purinergic P2Y receptor on Mg
2+
transport across intestinal epithelium-like Caco-2 monolayer. 3003 68
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