Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.4.3 (phospholipase C)
 18,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A total of 1002 samples comprising blood (n = 248), faecal swabs (n = 248), nasal swabs (n = 248) and deep vaginal swabs (n = 248) collected from the 248 sheep and 10 environmental samples of 10 sheep flocks were examined for the presence of pathogenic Listeria spp. Confirmation of the isolates was based on biochemical tests followed by phenotypic characterization by haemolysis on sheep blood agar, Christie Atkins Munch-Petersen (CAMP) test phosphatidylinositol-specific phospholipase C (PI-PLC) assay and phosphatidylcholine-specific phospholipase C (PI-PLC) assay. The isolates were subjected to genotypic characterization with the help of PCR assay for five virulence-associated genes, plcA, prfA, hlyA, actA and iap. The L. monocytogenes isolates were further subjected for multiplex-PCR-based serotyping. From 1002 samples screened, 16 (1.60%) were found positive for Listeria spp. Of these, seven samples (0.7%) were confirmed as L. monocytogenes and nine (0.9%) as L. innocua. All the seven isolates of L. monocytogenes were haemolytic, CAMP-positive, PI-PLC-positive, hlyA, pclA and prfA-positive by PCR, while only four isolates turned out to be PC-PLC-positive (opaque zone surrounding the growth). All the seven isolates of L. monocytogenes were serotyped as 4b. In conclusion, the PI-PLC assay and the virulence genes targeted PCR (plcA, prfA and hlyA plcA, prfA and actA genes for L. monocytogenes) hold a promise for rapid and reliable in vitro alternatives to in vivo pathogenicity tests.
Zoonoses Public Health 2009 Nov
PMID:Prevalence of Listeria spp including Listeria monocytogenes from apparently healthy sheep of Gujarat State, India. 1924 67

In an attempt to study the diversity and persistence of molecular subtypes of pathogenic Listeria spp. in a cheese factory at the La Mancha region of Spain, 43 samples were taken from incoming raw milk (cow's, ewe's, goat's and mixed species) and from certain food-contact and environmental surfaces before and/or after sanitation. Of these samples, 12 contained pathogenic Listeria. From the chromogenic agar plates corresponding to those, 46 phosphatidylinositol-specific phospholipase C-positive isolates were randomly taken for further analysis, including biochemical tests and pulsed-field gel electrophoresis (PFGE). They coincided in identifying all the 46 as Listeria ivanovii subsp. ivanovii, apparently a single PFGE type. Both ewe's and goat's raw milk batches from asymptomatic animals tested along the 6-month period persistently carried the same strain, which was also obtained from inner surfaces of raw milk truck tanks and the milk dump tank at the cheese factory. Biofilm-forming abilities of this L. ivanovii clone and interference against L. monocytogenes Scott A reference strain were tested, but failed to account for the clone's apparent pervasive presence.
Zoonoses Public Health 2010 Sep
PMID:Predominance and persistence of a single clone of Listeria ivanovii in a Manchego cheese factory over 6 months. 1948 91