Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.4.1 (phosphodiesterase)
 18,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Six different monoclonal antibodies were generated after immunization of mice with a partially purified enzyme preparation of rat liver, containing nucleotide-sugar hydrolase (NSH) I and II. These enzymes are also known under the names phosphodiesterase I and CMP-sialic acid hydrolase respectively [11]. In the enzyme-immunoassay the antibodies directed against NSH I displayed some cross-reactivity with the enzyme preparation of NSH II, and to a much lower extent the reverse was also true. Two antibodies, C and D highly reactive with NSH II and NSH I respectively, were used for immunocytochemical studies on sections of various rat tissues, which were known to contain high activities of both enzymes. Both antibodies were shown to be highly specific domain markers for different sides of the various cells. Antibody C was bound exclusively to the sinusoidal side of liver hepatocytes and to the basal side of cells from kidney tubule and epididymis. For antibody D the binding pattern was completely different, showing exclusive binding to the canalicular side of the hepatocytes and to the brush border membranes of kidney tubule cells, whereas in epididymis only binding to connective tissues was observed. Our studies clearly demonstrate, at least for liver and kidney, that NSH I and II are located at different cellular sides and that the monoclonal antibodies C and D can be used as domain markers for basal and apical sides of these cells respectively.
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PMID:Detection of different cellular sides in rat liver and kidney by two monoclonal antibodies raised against the nucleotide-sugar hydrolyzing enzymes phosphodiesterase I and CMP-sialic acid hydrolase. 298 96

Evidence is presented for the occurrence of two different non-specific nucleotide-sugar hydrolases in rat liver and other rat tissues. These two enzymes (I and II) were separated by chromatography on a 5'-AMP-aminohexyl-Sepharose column. Enzyme I is most probably identical with phosphodiesterase I (EC 3.1.4.1). Enzyme II appeared to be identical with an enzyme described in literature as 'CMP-sialic acid hydrolase' [Kean & Bighouse (1974) J. Biol. Chem. 249, 7813-7823], since almost all activity with CMP-N-acetylneuraminate as substrate was recovered in this enzyme fraction. CMP-N-acetylneuraminate was a poor substrate for Enzyme I, whereas deoxythymidine-5'-p-nitrophenyl phosphate and all nucleoside-diphosphosugars tested were good substrates for both Enzyme I and II. Therefore it is suggested that CMP-N-acetylneuraminate is used as an additional substrate to discriminate between the activities of Enzyme I and II in homogenates or membrane preparations. The various substrates appeared to be competitive inhibitors of each other, suggesting that, in each enzyme preparation, only one enzyme is responsible for the hydrolysis of the various substrates. The dissimilar properties of the two enzymes are substantiated by studying the subunit molecular masses (Enzyme I, 125 kDa; Enzyme II, 50-55 kDa), the sensitivity towards Triton X-100, Sarkosyl and sodium dodecyl sulphate and towards trypsin treatment. It is discussed whether the alpha-N-acetylglucosamine phosphodiesterase described by Varki & Kornfeld [(1981) J. Biol. Chem. 256, 9937-9943] is identical with one of the nucleotide-sugar hydrolases described here.
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PMID:On the presence of two non-specific nucleotide-sugar-hydrolysing enzymes in rat liver. 633 14