Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.4.1 (phosphodiesterase)
 18,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cotton (Gossypium hirsutum L.) contains a germin-like protein (GLP), GhGLP1, that shows tissue-specific accumulation in fiber. The fiber GLP is an oligomeric, glycosylated protein with a subunit size of approximately 25.5 kDa. Accumulation of GhGLP1 occurs during the period of fiber elongation [4-14 days post-anthesis (DPA)]. During early phases of fiber development (2-4 DPA), GhGLP1 localizes to cytoplasmic vesicles as shown by confocal immunofluorescent microscopy. In slightly older fibers (7-10 DPA), GhGLP1 localizes to the apoplast. In other plants, germins and GLPs have been reported to have enzymatic activities including oxalate oxidase (OxO), superoxide dismutase, and ADP-glucose pyrophosphatase. Cotton fiber extracts did not contain OxO activity, nor did intact fibers stain for OxO activity. A four-step purification protocol involving ammonium sulfate precipitation of a 1.0 M NaCl extract, ion-exchange chromatography on DEAE-Trisacryl M, lectin-affinity chromatography, and gel filtration chromatography resulted in electrophoretically pure GhGLP1. While 1.0 M NaCl extracts from 10-14 DPA fiber contained superoxide dismutase and phosphodiesterase activities, GhGLP1 could be separated from both enzyme activities by the purification protocol. Although a GLP accumulates in the cotton fiber apoplast during cell elongation, the function of this protein in fiber growth and development remains unknown.
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PMID:Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis. 1463 17

The presence of cotton ( Gossypium hirsutum L.) fiber transcripts coding for a germin-like protein (GLP) was revealed by differential display analysis in which early stages of cotton fiber development between a wild type line, Texas Marker-1 (TM1) and a near isogenic mutant, Naked Seed (N1) were compared. Transcripts of the cotton GLP ( GhGLP1) accumulated specifically in TM1, but did not accumulate in the mutant although the GhGLP1 gene was present in both lines. The deduced protein sequence of GhGLP1 is similar to Prunus persica auxin-binding proteins, a barley ADP-glucose pyrophosphatase/phosphodiesterase and two different classes of hydrogen peroxide-producing enzymes: wheat germin oxalate oxidase and moss extracellular Mn-superoxide dismutase. Cotton GLPs constitute a multigene family like those of Arabidopsis, rice, soybean, and barley. GhGLP1 transcripts accumulated to their highest levels during the period of fiber expansion, followed by a sharp decline when the rate of cell expansion decreased. While germins and GLPs appear to be involved in defense mechanisms in some plants, both biotic and abiotic stress down-regulated the expression of GhGLP1. Numerous functions have been proposed for dicot GLPs. However, to date, there is little direct evidence for how these proteins function in vivo. The association of maximal GhGLP1 expression with stages of maximal cotton fiber elongation suggests that some GLPs may be important for cell wall expansion.
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PMID:Cotton fiber germin-like protein. I. Molecular cloning and gene expression. 1464 17

Germin-like proteins (GLPs) are evolutionary conserved ubiquitous plant glycoproteins belonging to the cupin superfamily. A large number of GLP family members have been identified from different higher and lower plant species, and those have been classified into different subfamilies. Although three histidine residues (H) and one glutamate residue (E) in germin box B and C were conserved among all the GLP subfamily members, how the sequences of one subfamily member differ from the other is unclear. Progress in the field of genomics, transcriptomics, and proteomics has made it possible to understand the variation at gene level among different GLP members from diverse genera and also their biological significances. GLPs from different plant species were found to have various enzymatic properties including oxalate oxidase (OxO), superoxide dismutase (SOD), ADP glucose pyrophosphatase/phosphodiesterase (AGPPase), and polyphenol oxidase (PPO) activities. 'Omics' study demonstrated the expression as well as involvement of GLP family members in almost every part of higher plants as well as in lower plants. Additionally, GLPs from different species were reported to be involved in biotic as well as abiotic stresses and also in the growth and development. This review describes the present research status of GLPs from different plant species, their expressions, and functional significances. Sequence variation was detected among GLP subfamily members at the amino acid level, and based on the sequence variation and phylogenetic analyses, two new GLP subfamilies have been proposed in this review.
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PMID:Versatility of germin-like proteins in their sequences, expressions, and functions. 2617 51