Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.4.1 (
phosphodiesterase
)
18,767
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The synthesis of cyclic AMP by mouse peritoneal macrophages in response to stimulation by isoproterenol was studied as a function of drug concentration, incubation time and cell density. Cyclic AMP levels of macrophages increased 3 to 3.5 times over the control level 20 sec after the addition of 10(-3) M isoproterenol. Under these conditions the dose response could be followed down to an isoproterenol concentration of 10(-6) to 10(-7) M. When cell suspensions were inactivated as early as 1 sec after the addition of the drug, the increase in cyclic AMP was much greater (153 vs. 25 pmol/10(7) cells). Macrophage suspensions of high cell density were less responsive than those of low cell density. In the absence of any inhibitor of
phosphodiesterase
, the stimulatory effect of isoproterenol was always of short duration. The maximum effect of beta-adrenergic stimulation probably occurs in less than 1 sec at a cell density less than 2 x 10(6) cells/ml. The beta-blocking drug
Visken
abolished the observed effects.
...
PMID:Conditions for maximal synthesis of cyclic AMP by mouse macrophages in response to beta-adrenergic stimulation. 20 33
