Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.4.1 (phosphodiesterase)
 18,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The intracellular distribution of measles virus inclusion bodies in persistently infected human cells (AV3A1/MV) changed markedly following continuous exposure to 3', 5' cyclic adenosine monophosphate (cAMP). When assayed by immunofluorescence, the number of cells with intranuclear virus inclusions increased from 5 to 10% to 80 to 90% after exposure to 1 mM-cAMP for 4 days. Exposure of cells to cAMP also resulted in a twofold increase in the average number of inclusions in invaded nuclei. Similar but less pronounced changes occurred in cells treated with inducers of adenylate cyclase and an inhibitor of phosphodiesterase. Examination of cAMP-treated cells by electron microscopy indicated that viral inclusion bodies consisted of typical helical nucleocapsids. No evidence of nucleocapsids crossing the nuclear membrane (through nuclear pores) was found.
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PMID:Enhanced intranuclear expression of measles virus following exposure of persistently infected cells to cyclic AMP. 299 91

Conversion of acute measles virus infection to an indolent state has been achieved by treatment of infected cells of neural origin with agents that affect cyclic nucleotide metabolism. Striking results were obtained with papaverine, an inhibitor of cAMP phosphodiesterase that is capable of enhancing neural differentiation. In papaverine-treated cultures, decreased production of infectious virus was accompanied by selective disappearance of intracellular matrix proton, as detected by immunofluorescence. Viral nucleocapsid protein was enhanced in the cytoplasm while three other structural proteins--polymerase, hemagglutinin, and fusion protein--showed little change in distribution or intensity of staining. These results were specific for cells of neural origin and not observed in CV-1 or Vero cultures. cAMP, dibutyryl cAMP, 8-bromo-cAMP, and isobutylmethylxanthine all inhibited replication of virus but less so than did papaverine. Inhibition of virus replication by any of these agents was rapidly reversible, either by removal of the agent or by addition of cGMP to the culture medium and was accompanied by reappearance of the matrix protein. These results suggest that measles virus replication in neural cells depends on host factors, particularly those affecting endogenous cAMP and cGMP. Viral persistence may thus be related to the state of neural differentiation. This model system may yield information on mechanisms of recrudescence observed in some chronic diseases of the nervous system.
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PMID:Reversible repression and activation of measles virus infection in neural cells. 628 Jan 93

The survival rate of mice with exposure of the whole body (700 cGy) was hardly changed by one dose as well as several doses of the phosphodiesterase inhibitor Amantadine and the interferon inductor measles vaccine. However, the survival rates were increased by one administration of L-dopa or by the long-term therapy using L-dopa at 700 and 900 cGy. The survival rates were also increased at 700 and 900 cGy if, after the exposure, thymus factor isolated from calf thymus was three times applied to the animals. The increased survival rates gained by using L-dopa and thymus factor are correlated with the determined leukocyte values.
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PMID:[The influence of L-dopa and of thymus fraction on the survival rate of whole-body-irradiated mice]. 675 89