Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.31.1 (
micrococcal nuclease
)
2,818
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Soluble chromatin was prepared from rat testes after a brief
micrococcal nuclease
digestion. After adsorption onto hydroxylapatite at low ionic strength, the histone H1 subtypes were eluted with a shallow salt gradient of 0.3 M NaCl to 0.7 M NaCl.
Histone H1t
was eluted at 0.4 M NaCl, while histones H1a and H1c were eluted at 0.43 M NaCl and 0.45 M respectively. The extreme divergence of the amino acid sequence of the C-terminal half of
histone H1t
, the major DNA binding domain of histone H1, from that of the somatic consensus sequence may contribute to the weaker interaction of
histone H1t
with the rat testis chromatin. Further,
histone H1t
was not phosphorylated in vivo in contrast to histone H1a and H1c, as is evident from the observation that
histone H1t
lacks the SPKK motif recognized by the CDC-2kinase or the RR/KXS motif recognized by protein kinase A.
...
PMID:Testis-specific histone (H1t) is not phosphorylated and has a weak interaction with chromatin. 800 17
