Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.31.1 (
micrococcal nuclease
)
2,818
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Chromatin from rat pituitary tumor (GH3) cells was fractionated into transcriptionally active and inactive domains. When the various chromatin fractions were assayed for their content of
growth hormone
gene sequences, it was found that these sequences were highly enriched in those chromatin fractions most sensitive to
micrococcal nuclease
. Fraction S1 showed the highest enrichment in
growth hormone
genes and was impoverished in histones H3 and H4. The distribution of specifically bound thyroid and glucocorticoid receptors in chromatin fractions enriched and depleted in
growth hormone
gene sequences was also examined. Both thyroid and glucocorticoid receptors are enriched to different extents in transcriptionally active chromatin. Within active chromatin, both types of receptors exist in more than one molecular form.
...
PMID:Distribution of thyroid hormone receptors, glucocorticoid receptors, and growth hormone gene sequences in chromatin from cultured rat pituitary cells. 630 41
I have measured the effect of hormones and other regulatory factors present in the serum component of the culture medium on the levels of
growth hormone
and prolactin mRNAs in rat pituitary (GH4) cells. Hybridization of cytoplasmic RNA with
growth hormone
or prolactin cDNA clones indicate that serum depletion reduces significantly the amount of these two mRNAs. The localization of these two genes in chromatin was also analysed using
micrococcal nuclease
as a probe. At intermediate levels of digestion (about 10% of the input A260 released into a soluble supernatant S1), the bulk of both
growth hormone
and prolactin genes are rapidly solubilized by the nuclease and appear in the soluble supernatant S1. Nevertheless, at low levels of digestion (less than 4% of the input A260 released into S1) the
growth hormone
gene remains exquisitively sensitive to
micrococcal nuclease
while the sensitivity of the prolactin gene is reduced considerably. When one compares the distribution of
growth hormone
and prolactin genes in chromatin fractions differing in nuclease sensitivity and derived from cells grown in control medium or in depleted medium, it appears that markedly reduced transcriptional activity of the prolactin gene shows no correlation with altered chromatin structure. On the other hand, the chromatin structure of the
growth hormone
gene is significantly altered when transcription is markedly reduced.
...
PMID:Modulations of prolactin and growth hormone gene expression and chromatin structure in cultured rat pituitary cells. 668 34
