Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.31.1 (micrococcal nuclease)
 2,818 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mouse thymidine kinase negative (tk-) L-cells were cotransformed with two different kappa light chain genes (cloned from mouse myeloma) and the tk gene from Herpes simplex virus I. (Transformation is defined as change in the genotype by introduction of foreign DNA.) About 80% of the tk+ -transformants contained varying amounts of transferred kappa light chain sequences, one transformant about 150 copies per genome. The transferred immunoglobulin genes appear to be organized in a nucleosomal substructure, as deduced from digestion experiments with micrococcal nuclease. In situ hybridization experiments revealed, that the transferred genes are not distributed randomly across the chromosomes of the recipient cell. Instead they are clustered at one or a few chromosomal locations.
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PMID:Localization in mouse-L-Cell chromosomal sites of transferred immunoglobulin genes. 628 Sep 34

We have utilized the Sanders salt fractionation technique (Sanders, M. M. (1978) J. Cell Biol. 79, 97-109) to analyze the products of micrococcal nuclease digestion of adult chicken erythrocyte nuclei. By dot-blot hybridization with specific gene probes, it is found that nucleosomes from the globin gene domain, including a region extending to about 10 kilobase pairs 5' to the beta p gene are selectively enriched in the fractions eluted at low salt. In contrast, a single copy sequence located at about 10 kilobase pairs 5' to the beta p gene was concentrated in the less salt-soluble fractions. The vitellogenin and ovalbumin genes, which are never expressed in erythroid tissues, are also concentrated in the less salt-soluble fractions. Some more generally expressed genes (histone H4, thymidine kinase) appear to be more uniformly distributed. The low salt fractions are depleted in H1/H5, enriched in high mobility group 14 and 17, and contain somewhat more highly acetylated histones.
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PMID:Differential salt fractionation of active and inactive genomic domains in chicken erythrocyte. 673 42