Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.31.1 (
micrococcal nuclease
)
2,818
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recent data revealed that
DEK
associates with splicing complexes through interactions mediated by serine/arginine-repeat proteins. However, the
DEK
protein has also been shown to change the topology of DNA in chromatin in vitro. This could indicate that the
DEK
protein resides on cellular chromatin. To investigate the in vivo localization of
DEK
, we performed cell fractionation studies, immunolabeling, and
micrococcal nuclease
digestion analysis. Most of the
DEK
protein was found to be released by DNase treatment of nuclei, and only a small amount by treatment with RNase. Furthermore,
micrococcal nuclease
digestion of nuclei followed by glycerol gradient sedimentation revealed that
DEK
co-sedimentates with oligonucleosomes, clearly demonstrating that
DEK
is associated with chromatin in vivo. Additional chromatin fractionation studies, based on the different accessibilities to
micrococcal nuclease
, showed that
DEK
is associated both with extended, genetically active and more densely organized, inactive chromatin. We found no significant change in the amount and localization of
DEK
in cells that synchronously traversed the cell cycle. In summary these data demonstrate that the major portion of
DEK
is associated with chromatin in vivo and suggest that it might play a role in chromatin architecture.
...
PMID:Subcellular localization of the human proto-oncogene protein DEK. 1133 57
