Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.31.1 (micrococcal nuclease)
 2,818 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In recent years several tissues have been found to contain the components of the renin-angiotensin system. Locally synthesized renin could conceivably function in an endocrine, paracrine or autocrine manner. Isolated left ventricular cardiac myocytes of the rat were examined in the present study and renin and angiotensin II were detected in these cells. This observation suggests the existence of a functioning renin-angiotensin system in the heart and, more particularly, in the left ventricular cardiac myocyte. Additional evidence, derived from micrococcal nuclease and deoxyribonuclease I treatment of angiotensin II-exposed chromatin, suggests that angiotensin II could subserve an intracellular function in some of its cells of synthesis.
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PMID:The myocardial intracellular renin-angiotensin system. 381 63

When isolated rat hepatic nuclei or bovine thymus nuclei were incubated with 125I-angiotensin II (ANG II) in the presence or absence of cold hormone, displaceable binding was consistently detected in micrococcal nuclease generated fragments Nanomolar concentrations of ANG II produced detectable displacement. Little or no specific binding was found when nuclei were first digested and then treated with hormone, suggesting that ANG II solubilizes its own receptor. The binding moiety was partially purified by DNP gel electrophoresis. These studies indicate the existence in chromatin of high affinity receptors for ANG II, and further suggest that hormone binding to these receptors produces conformational changes in chromatin similar to those seen during enhanced transcriptional activity. Thus, the present studies suggest the existence of functional intracellular renin-angiotensin systems.
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PMID:Angiotensin II receptors in chromatin. 610 Jul 40