Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.31.1 (micrococcal nuclease)
 2,818 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Urea and dithiothreitol can decondense the chromatin in some of rat sperm heads. By this treatment, we have observed that in the nuclei of rat sperm the chromatin is organized into two morphologically distinct portions, namely: the compact chromatin rods of about 450 to 1,000 A thick, and the interlacing fibers about 250-290 A in thickness. When these treated sperm are further digested with micrococcal nuclease, the small fibers disappear, whereas the chromatin rods are still present in the "urea-nuclease pellet." From the available evidence, we suggest that the chromatin rods represent the highly packed nucleoprotamine, whereas the small fibers represent the more loosely organized nucleohistone.
Anat Rec 1981 Oct
PMID:Electron microscopic studies of rat sperm heads treated with urea, dithiothreitol, and micrococcal nuclease. 731 26