Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have generated and purified a new recombinant baculovirus with expanded host range. AcNPV DNA and BamHI-digested BmNPV DNA were co-transfected into Spodoptera frugiperda SF21 cells. Progeny viruses were used to infect BmN cells, which are normally resistant to AcNPV infection, in order to screen for recombinant viruses with cross-infectivity. A recombinant virus was isolated by plaque purification and analyzed. This isolate was able to infect, replicate and produce polyhedrin in both the SF21 and BmN cells. DNA restriction endonuclease analysis showed that it was a hybrid (HyNPV) of AcNPV and BmNPV. Co-transfection of this HyNPV virus with a transfer vector containing a ligninase H8 gene insert into SF21 cells yielded a recombinant baculovirus expressing the ligninase. When this recombinant baculovirus was used to infect either SF21 or BmN cells, ligninase proteins could be found in the lysed cells as well as in the cell culture media. We have thus demonstrated that this hybrid virus can be utilized in the generation of recombinant baculovirus expressing foreign proteins in two host cells which normally can not be infected by AcNPV nor BmNPV.
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PMID:Expression of a Ligninase by a New Hybrid Baculovirus with Expanded Host Range. 1221 9

Spodoptera frugiperda nuclear polyhedrosis virus (SfMNPV was obtained from four different sources and each isolate was cultured in IPLB-SF21 cells. Viral DNA from each isolate was analyzed with EcoR1, Xho1, and BamH1 restriction endonucleases. All four isolates could be distinguished on the basis of minor differences in EcoR1 patterns. Submolar DNA restriction fragments were seen in all isolates indicating that each isolate consisted of a mixture of different genotypes. To investigate this, two of the isolates were plaque purified. Of the total of 13 clones isolated, the DNA from 5 of these had distinctly different EcoR1 restriction endonuclease patterns. The five cloned variants were further analyzed with Xho1, BamH1, and Sac1. Three of the five clones had fragments which comigrated with submolar fragments seen in the wild isolates. Thus, the submolar fragments observed in digests of the DNA from two wild isolates of SfMNPV were due to a mixture of genetic variants.
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PMID:Investigation of genetic heterogeneity in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified variants. 1863 67