Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new type II restriction
endonuclease
designated OLI:I has been partially purified from the halophilic bacterium Oceanospirillum linum 4-5D. OLI:I recognizes the interrupted hexanucleotide palindrome 5'-
CACNN
NNGTG-3' and cleaves it in the center generating blunt-ended DNA fragments.
...
PMID:OliI, a unique restriction endonuclease that recognizes the discontinuous sequence 5'-CACNN NGTG-3'. 1123 7
Giardia duodenalis
is one of the most common and important protozoan parasites of the gastrointestinal tract in humans and animals, especially in developing countries. The purpose of this study was determining prevalence of
Giardia
genotypes specially zoonosis genotypes in sheep and goat in eastern of iran slaughterers.This cross-sectional study was conducted during April to November 2019. 300 fecal samples were collected from the rectum of sheep and goats. The samples were subjected to DNA extraction after sucrose gradient purification. A fragment of the glutamate dehydrogenase gene (
gdh
) was amplified by semi-nested PCR and genotype diagnosis was performed by digestion of the secondary PCR product with restriction enzymes
Rsa
I and
Nla IV
. The prevalence of
Giardia
was found as (274/300) by the molecular method. Restriction
endonuclease
digestion of the nested-PCR product showed; among 274 positive isolates, 95 were typed as assemblage E, 15 as assemblage B, 87 assemblage AI, 45 assemblage AII, and 32 assemblege C. In this study, frequency of different assemblages of
G. duodenalis
was determined in sheep and goats by
gdh
gene and PCR-RFLP method. Same of other studies, assemblage E was dominant genotype in sheep and goats. Isolation of zoonotic assemblages as AI, AII, and
BIII
showed that sheep and goats should be considered as a source for human infection.
...
PMID:Frequency of different genotypes of
Giardia duodenalis
in slaughtered sheep and goat in east of iran. 3280 15
