Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RNA sequencing (RNA-Seq)-based gene expression analysis is applicable to a wide range of biological purposes in various species. Reverse transcription quantitative PCR (RT-qPCR) is also used to assess target gene expression utilizing stably expressed reference genes as internal control under a given set of conditions. However, investigations of the reference genes for RT-qPCR normalization in the process of somatic embryogenesis (SE) initial dedifferentiation in
Gossypium hirsutum
are rarely reported. In this study, on the basis of our previous transcriptome data of three different induction stages during SE initial dedifferentiation process in four
G. hirsutum
cultivars that have different SE capability, 15 candidate genes were selected during SE initial dedifferentiation process, and their expression stability was evaluated by geNorm, NormFinder, and BestKeeper. The results indicated that the two genes of
endonuclease
4
(
ENDO4
) and
18S ribosomal RNA
(
18S rRNA
) showed stable expression in the four different
G. hirsutum
cultivars, endowing them to be appropriate reference genes during three induction stages in the four cotton cultivars. In addition, the stability and reliability of the two reference genes of
ENDO4
and
18S rRNA
were further verified by comparing the expressions of
auxin-responsive protein 22
(
AUX22
) and
ethylene-responsive
transcription factor 17
(
ERF17
) between RT-qPCR results and the RNA-seq data, which showed strong positive correlation coefficient (R
2
= 0.8396-0.9984), validating again the steady expression of
ENDO4
and
18S rRNA
as the reliable reference genes. Our results provide effective reference genes for RT-qPCR normalization during SE process in different
G. hirsutum
cultivars.
...
PMID:Screening the Reference Genes for Quantitative Gene Expression by RT-qPCR During SE Initial Dedifferentiation in Four
Gossypium hirsutum
Cultivars that Have Different SE Capability. 3126 92
