Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.30.2 (
endonuclease
)
18,621
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The histone chaperone SET is required for transcription of chromatin templates by RNA polymerase Pol II (Pol II) in vitro. Here we uncover a positive role for SET in dislodging
DEK
and PARP1, which restrict access to chromatin in the absence of SET and the PARP1 substrate NAD(+). SET binds chromatin, dissociating
DEK
and PARP1 to allow transcription in the absence of NAD(+). In the absence of SET, depletion of
DEK
restores chromatin accessibility to
endonuclease
but does not permit Mediator recruitment or transcription. In the presence of NAD(+), PARP1 poly(ADP-ribosyl)ates and evicts
DEK
(and itself) from chromatin to permit Mediator loading and transcription independent of SET. An artificial
DEK
variant resistant to SET and PARP1 represses transcription, indicating a requirement for
DEK
removal. Therefore, SET,
DEK
and PARP1 constitute a network governing access to chromatin by the transcription machinery.
...
PMID:SET and PARP1 remove DEK from chromatin to permit access by the transcription machinery. 1752 93
