Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The clinical findings relating to 11 patients in Hong Kong (HK) and to 43 patients described elsewhere, all with Streptococcus zooepidemicus septicaemia, are reviewed. There was a particular association with cardiovascular disease (27%) with seven cases of endocarditis, three of abdominal aortic aneurysm and two of deep venous thrombosis. Associations not previously reported included two cases of pharyngitis and two patients with persistent post-operative fever. The overall mortality was 22%. Both human and porcine strains of S. zooepidemicus from HK did not hydrolyse aesculin in contrast to the aesculin-positive biotypes reported previously. HK strains also had very mucoid colonies and capsules of hyaluronic acid were seen in electron micrographs. Samples of chromosomal DNA, extracted by means of HindIII restriction endonuclease, of strains from human beings and pigs were identical. The MIC of penicillin for all strains was less than or equal to 0.03 mg/l but the MBC for all was greater than 32 mg/l. Penicillin alone is generally sufficient for cure but combination with an aminoglycoside may be indicated in seriously ill patients. In our locality, pigs were incriminated as a possible source of human infection whereas consumption of contaminated dairy products is important elsewhere.
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PMID:Streptococcus zooepidemicus (Lancefield group C) septicaemia in Hong Kong. 227 71

GCL3, a beta-lactamase-producing, penicillin resistant strain of Neisseria gonorrhoeae isolated in Toulouse (France), and GCL51, a penicillin susceptible strain, were examined for their plasmid content. Agarose-gel electrophoresis following or not ultracentrifugation in a cesium chloride-ethidium bromide gradients, revealed, for both strains a 3.9-kilobase (kb) (2.6 megadaltons) cryptic plasmid. Penicillin resistant strain GCL3 also contained a 37 kb (24.5 megadaltons) and a 7.3 kb (4.8 megadaltons) plasmid. Transformation studies showed that the gene responsible for beta-lactamase production was carried by the 7.3-kb plasmid. E. coli cells transformed for ampicillin resistance by plasmid DNA from GCL3, contained a single 7.3 kb-plasmid. The restriction endonuclease cleavage map obtained for this plasmid indicated that it is closely related to a penicillin R plasmid previously described in a strain isolated in the USA. The first isolation in the Midi-Pyrenees area of a beta-lactamase-producing strain proved the necessity of a rigorous surveillance of N. gonorrhoeae strains isolated in the world.
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PMID:[Isolation and characterization of a beta-lactamase-specifying plasmid in a strain of "neisseria gonorrhoeae" (author's transl)]. 627 Oct 37

Previous studies have suggested that relatively penicillin-resistant (RPR) capsular group 9L strains in western Canada may be clonally related. To test this hypothesis, restriction fragment length polymorphisms (RFLPs) were examined using DNA probes for pspA and a newly recognized pneumococcal genetic element, IS1167. Penicillin-binding proteins (PBPs) and PBP genes from representative strains were also studied. All RPR type 9L strains demonstrated an identical RFLP when probed with IS1167, and 12 of 14 RPR strains had the same RFLP when examined with pspA. Amplification of pspA by polymerase chain reaction and restriction endonuclease digestion showed that the 9L strains had common DNA fragments not identified in any of the penicillin-susceptible strains. The 9L strains apparently have a low-affinity PBP 2B distinct from those of other capsular types. These data derived from new genetic markers and PBP analysis strongly support a clonal origin of RPR type 9L pneumococci of western Canada.
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PMID:DNA polymorphisms and variant penicillin-binding proteins as evidence that relatively penicillin-resistant pneumococci in western Canada are clonally related. 884 37