Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The nucleotide sequences in the replicative form (duplex) of phiX174 DNA around six sites cut by Hga I, a restriction endonuclease from Haemophilus gallinarum, have been compared. The enzyme produces a staggered cleavage resulting in a pentanucleotide 5'-terminal extension. The sequences within and immediately surrounding the pentanucleotide cleavage site have no obvious relationship. However, the sequence 5'-G-A-C-G-C-3' 3'-C-T-G-C-G-5' occurs five nucleotide pairs to the left of the cut in the upper strand and 10 nucleotide pairs to the left of the cut in the lower strand and, therefore, is believed to constitute the recognition site. This is a member of the class of restriction endonucleases in which recognition and cleavage sites lack 2-fold rotational symmetry. The method used to define the cleavage site is of general applicability.
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PMID:Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I). 26 84

A site-specific restriction endonuclease Fnu4H I isolated from Fusobacterium nucleatum 4H recognizes the DNA nucleotide sequence 5'G C N G C-3'/3'-C G N C G-5' and cleaves as indicated by the arrows.
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PMID:A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC. 42 88

The genes encoding the KpnI restriction and modification (R-M) system from Klebsiella pneumoniae, recognizing the sequence, 5'-GGTAC decreases C-3', were cloned and expressed in Escherichia coli. Although the restriction endonuclease (ENase)- and methyltransferase (MTase)-encoding genes were closely linked, initial attempts to clone both genes as a single DNA fragment in a plasmid vector resulted in deletions spanning all or part of the gene coding for the ENase. Initial protection of the E. coli host with MTase expressed on a plasmid was required to stabilize a compatible plasmid carrying both the ENase- and the MTase-encoding genes on a single DNA fragment. However, once established, the MTase activity can be supplied in cis to the kpnIR gene, without an extra copy of kpnIM. A chromosomal map was generated localizing the kpnIR and kpnIM genes on 1.7-kb and 3.5-kb fragments, respectively. A final E. coli strain was constructed, AH29, which contained two compatible plasmids: an inducible plasmid carrying the kpnIR gene which amplifies copy number at elevated temperatures and a pBR322 derivative expressing M.KpnI. This strain produces approx. 10 million units of R.KpnI/g of wet-weight cells, which is several 1000-fold higher than the level of R.KpnI produced by K. pneumoniae. In addition, DNA methylated with M.KpnI in vivo does not appear to be restricted by the mcrA, mcrB or mrr systems of E. coli.
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PMID:Cloning the KpnI restriction-modification system in Escherichia coli. 199 32

PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognition sites, respectively. Seventy-two strains of Pseudomonas, Clostridium, Escherichia coli, Shigella, Proteus and Saccharomyces were screened for the presence of site-specific endonucleases. Here we describe the PaeI restriction enzyme found in Pseudomonas aeruginosa; other data will be published elsewhere. Earlier Hinkle and Miller isolated from P. aeruginosa a PaeR7 restriction endonuclease recognizing and cleaving a sequence 5'-C reduced TCGAG-3' (1). Sequence analysis of DNAs cleaved by PaeI shows that the enzyme is the isoschizomer of SphI (2).
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PMID:A site-specific endonuclease from Pseudomonas aeruginosa. 299 50

Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the sequence 5'-GCATG C-3'. Another isolated enzyme PaeII is an isoshizomer of SmaI and cleaves DNA in a fragment 5'-CCC GGG-3'. The use of PaeI and PaeII enzymes in genetical engineering and their advantages are discussed.
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PMID:[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa]. 302 9

A site-specific endonuclease, SciNI, has been partially purified from the plant pathogen Spiroplasma citri. The enzyme recognizes the sequence 5'-G-C-G-C-3' and cleaves between the first G and C. 3'-C-G-C-G-5' SciNI is an isoschizomer of HhaI, but generates DNA fragments with 5' rather than 3' single-stranded protrusions.
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PMID:Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri. 627 58

The binding properties of the type II restriction endonuclease SalGI to the plasmid DNA pGW 10 has been investigated by electron microscopic studies. Samples were spread by the BAC technique. In the presence of magnesium, SalGI binds as dimers and tetramers to the specific recognition site 5'-G-T-C-G-A-C-3' and with lower rate to the sequence 5'-G-T-C-A-A-C-3', which represents the recognition site of the restriction endonucleases Hind II and Hinc II.
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PMID:[Electron microscopy studies of DNA complexes with restriction endonuclease SalGI]. 630 17

The cleavage site for the restriction endonuclease EcoRV has been found to be 5'-GAT/ATC-3', rather than 5'- GATAT /C-3' as reported earlier by Kholmina et al. [ Dokl . Akad . Nauk . 253 (1980) 495-497].
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PMID:The cleavage site for the restriction endonuclease EcoRV is 5'-GAT/ATC-3'. 632 9

PpeI is a type II restriction endonuclease isolated from cyanobacterial strain Phormidium persicinum. The endonuclease PpeI, an isoschizomer of ApaI, recognizes the hexanucleotide sequence (5'-GGGCC/C-3') and cleaves, after the second C, producing four nucleotide 3'-cohesive ends.
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PMID:Isolation and characterization of the restriction endonuclease PpeI from Phormidium persicinum. 873 23

Screening of thermophilic bacterial strains revealed a strain containing three site-specific endonucleases: BspLAI, BspLAII, and BspLAIII. These endonucleases were purified to functional purity by sequential chromatography. Recognition sites, DNA cleavage sites, and some properties of the endonucleases were determined. BspLAI recognizes the sequence 5-GCG/C-3 on the DNA molecule and is an isoschizomer of endonuclease HhaI. BspLAII recognizes the sequence 5-TT/CGAA-3 and is an isoschizomer of AsuII. BspLAIII recognizes site 5-A/AGCTT-3 and is an isoschizomer of endonuclease HindIII. All the three enzymes exhibit maximal activity at 55 degrees C. The optimal buffer is MRB, pH 7.4. They retain activity on storage for 3 weeks at room temperature and thus are highly stable.
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PMID:Three site-specific endonucleases from thermophilic strain Bacillus species LA are isoschizomers of HhaI, AsuII, and HindIII. 931 22


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