Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.30.2 (endonuclease)
 18,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The lack of transcriptional regulation in trypanosomatids suggests the presence of distinct posttranscriptional mechanisms to control differential gene expression. In fact, the stability of S-phase specific mRNAs in these parasites is determined primarily by the presence of the octanucleotide sequence (C/A)AUAGAA(G/A) in the UTRs of the transcripts. Here, the characterization of LdCSBP is reported, which specifically binds to the octanucleotide containing RNA. The LdCSBP protein contains multiple putative functional domains, including two types of ubiquitin binding domains (UBA and CUE), two CCCH-type Zn-finger motifs probably responsible for specific RNA binding activity and a speculative endonuclease domain SMR. Interestingly, the protein is covalently modified through ubiquitination. This observation and the occurrence of multiple ubiquitin binding domains in the protein raise the possibility of regulation of the activity of LdCSBP by ubiquitination.
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PMID:mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is covalently modified by ubiquitination. 1755 72

In nearly complete absence of transcriptional regulation, messenger RNA (mRNA) turnover mediated through specific cis-elements plays a predominant role in the control of differential gene expression for the disease causing trypanosomatid parasites. In these organisms, the periodic accumulation of S-phase messages during cell cycle is determined by the presence of one or more copies of a conserved CAUAGAAG octanucleotide motif in the untranslated regions of mRNAs. In our previous studies, a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani was characterized, which binds specifically to the octamer-containing RNAs via its uniquely arranged CCCH-type Zn fingers and degrades them through its small MutS-related (Smr) endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Interestingly, the protein is modified by the incorporation of a monoubiquitin residue, and the posttranslational modification inhibits its riboendonuclease activity. However, the mechanism of such inhibition was previously unknown. Here, we establish that the CCCH-type Zn finger domain is the site of ubiquitination in LdCSBP and the interaction of CUE domain of the protein with the ubiquitinated Zn finger domain is responsible for inhibition of its riboendonuclease activity. The findings elucidate an inhibitory mechanism of RNA cleavage through ubiquitination-mediated intramolecular interaction among domains of the enzyme. Furthermore, the riboendonuclease activity is inhibited by anti-leishmanial drug paromomycin suggesting that the regulation of RNA metabolism could be a target of the drug.
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PMID:Ubiquitination-mediated interaction among domains is responsible for inhibition of RNA endonuclease activity of mRNA cycling sequence binding protein from L. donovani (LdCSBP). 2490 31